Biuret and protein reaction principle
The principle of the reaction between biuret and protein was that biuret could react with copper sulfuric acid to form a purple substance. This might be caused by the peptidic bond structure in the biuret molecules. The nitrogen atom of the peptidic bond would form a purple complex with Cu2 + ions. Because there was also a peptidic bond structure in the protein, such a reaction would also occur. We called the purple reaction between the compound containing the peptidic bond structure and copper sulfuric acid solution "biuret reaction". In other words, as long as the molecules contained a peptidic bond, this characteristic purple reaction would generally occur. It wasn't that biuret reacted directly with the protein, but the reaction between the peptidic bond in the protein and copper ions. It was the same reaction type as biuret and copper ions. It was a " biuret reaction." When carrying out experiments related to the biuret reaction, biuret reagent A was a strong base, and biuret reagent B was copper sulfuric acid. When using it, add A to the protein solution first, shake it up, and then add B. Strong base and copper sulfuric acid can denature the protein, but this does not affect the purple reaction, because the essence of protein degeneration is that the spatial structure is destroyed, causing it to settle and lose its physiological activity. However, the bond structure and the sequence of the protein chain are generally not destroyed. Read more exciting novels for free
Reaction conditions of protein and biuret reagent
The reaction condition of the protein and biuret reagent was room temperature (no heating was needed). During the reaction, solution A (0.1 g/mL of either lithium or potassium) was added first, followed by solution B (0.01 g/mL of copper sulphate), and the reaction needed to be carried out in an acidic environment. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Biuret reagent test protein reaction conclusion
When the biuret reagent was used to detect protein, when the base contained a peptidic bond (a peptidic bond), the copper in the test solution would coordinate with the peptidic bond, and the complex would turn purple. The color of the purple complex produced by the reaction was directly proportional to the concentration of the protein, and it had nothing to do with the molecular weight of the protein and the composition of the protein. The concentration could be analyzed by a prismatic method, and the wave-length in the ultraviolet-visible spectrum was 540mn. The sensitivity of the identification reaction was 5 - 160g/ml, and the identification reaction protein unit was 1 - 10g. Moreover, the compound molecules that could produce a purple reaction with the biuret reagent contained at least two peptidic bonds, so dipeptides could not be tested with it. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Is the biuret reaction a qualitative or quantitative reaction?
The biuret reaction can be used to determine the quality or quantity of the protein. Biuret reagent could react with protein and form a purple complex in an acidic solution. The color was proportional to the concentration of the protein. Therefore, the existence of the protein could be detected by this reaction, and it could also be measured. However, biuret reagents could not be used to detect dipeptides because the reaction required the test substance to have at least two peptidic bonds. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Is there a biuret reaction in the presence of vitamins C?
There was no biuret reaction in the presence of vitamin C. The biuret reaction was a reaction of a protein under the action of a biuret reagent, which would produce a purple complex. However, the biuret reaction could not occur due to the lack of structural properties of the protein. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
The opsonizing reaction of the protein protein
If the patient has an allergic constitution and is allergic to the protein, it may induce an allergic reaction after use, causing symptoms such as itchy skin, redness, swelling, and even difficulty breathing. If you don't follow the doctor's advice and take the medicine on time, it may stimulate the stomach and intestines, causing discomfort such as loss of appetite, abdominal distension, and abdominal pain. Since the protein was mainly digested by the liver and kidneys, excessive use would increase the burden on the liver and kidneys. The kidneys might have oliguria, anuria, lumbago and other symptoms. The liver might have loss of appetite, abdominal distension, abdominal pain, yellow skin, nausea and other symptoms. It might also lead to increased burden on the heart, chest tightness, short breath and other discomfort. The novel " Watching the Moon on Fish Island " is equally exciting. Everyone is welcome to click and read it!
Is C-reaction protein pre-protein?
No, C-reaction protein is not pre-protein. C-reaction protein was an acute phase reaction protein synthesized by liver cells and released into the blood. When the body was invaded by microorganisms, tissue damage, or self-inflammation, the liver cells would synthesize it. It was an extremely sensitive indicator of the body's acute phase reaction. Prealbumin was synthesized by liver cells, and measuring its concentration in blood plasma was of clinical significance for understanding the malnutrition, liver incompetence, low protein, and transferring of serum. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Is the biuret reaction a qualitative or quantitative test method?
The biuret reaction could be both a qualitative test method and a quantitative test method. From a qualitative point of view, the biuret reaction can be used to identify the presence of a protein. Because the biuret reaction was a unique reaction between a protein and a protein, when a purple or blue-purple complex was detected, it could indicate the presence of a protein (except for dipeptides, because the reaction required the test substance to have at least two peptidic bonds). From a quantitative point of view, the color of the purple complex produced by the biuret reaction was directly proportional to the concentration of the protein. Therefore, the protein concentration could be analyzed by the chromometer. Within a certain range, it complied with the Bill's Law, and the protein content could be determined. The range of measurement was 1 - 10 milligrams of protein, which was suitable for the determination of the protein content above 0.5 milligrams. However, the biuret method had the disadvantages of low accuracy and large sample volume. Moreover, the TrIs buffer, which was an acidic buffer or a peptide-based buffer, would interfere with the determination because they produced a positive color reaction. At the same time, copper ions were easily reduced and sometimes red precipitations appeared. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Reaction of Bromate with a protein
It could react with the protein in the wheat flour to enhance the strength and elasticity of the flour, forming a good network of flour, thus significantly improving the baking effect of flour. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Principle of Allergen Reaction
Allergy reactions are caused by some people being overly sensitive to the substances (allergens) they come into contact with. When a sensitive individual comes into contact with a foreign, harmless substance (an allergy), the immune system will overreact and the body will produce a type of protein specific to the allergy. These IgE-bound to the high-affinity receptor on mast cells and basophils. When the allergy entered the body again and met with these bound molecules, the form of the immune receptor would change from a dimmer to a monolith. This change in form would lead to the exposure of the protein sites of the relevant signaling pathways, which would activate the downstream signaling pathways, causing allergic reactions such as vasodilation and bronchiectomy. In severe cases, it could cause allergic shock. In an allergic reaction, the affected organs or tissues will be infiltrated by a large number of inflammatory cells (especially those that are not activated), and a high concentration of IgE will appear in the serum. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
The reaction between urine and biuret reagent before exercise
There was no protein in urine, so there was no purple reaction between urine and biuret reagent before exercise. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>