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Is the biuret reaction a qualitative or quantitative reaction?

Is the biuret reaction a qualitative or quantitative reaction?

2026-08-15 10:59
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The biuret reaction can be used to determine the quality or quantity of the protein. Biuret reagent could react with protein and form a purple complex in an acidic solution. The color was proportional to the concentration of the protein. Therefore, the existence of the protein could be detected by this reaction, and it could also be measured. However, biuret reagents could not be used to detect dipeptides because the reaction required the test substance to have at least two peptidic bonds. Read more exciting novels for free

Biuret and protein reaction principle

The principle of the reaction between biuret and protein was that biuret could react with copper sulfuric acid to form a purple substance. This might be caused by the peptidic bond structure in the biuret molecules. The nitrogen atom of the peptidic bond would form a purple complex with Cu2 + ions. Because there was also a peptidic bond structure in the protein, such a reaction would also occur. We called the purple reaction between the compound containing the peptidic bond structure and copper sulfuric acid solution "biuret reaction". In other words, as long as the molecules contained a peptidic bond, this characteristic purple reaction would generally occur. It wasn't that biuret reacted directly with the protein, but the reaction between the peptidic bond in the protein and copper ions. It was the same reaction type as biuret and copper ions. It was a " biuret reaction." When carrying out experiments related to the biuret reaction, biuret reagent A was a strong base, and biuret reagent B was copper sulfuric acid. When using it, add A to the protein solution first, shake it up, and then add B. Strong base and copper sulfuric acid can denature the protein, but this does not affect the purple reaction, because the essence of protein degeneration is that the spatial structure is destroyed, causing it to settle and lose its physiological activity. However, the bond structure and the sequence of the protein chain are generally not destroyed. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

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2026-07-06 02:06

Is there a biuret reaction in the presence of vitamins C?

There was no biuret reaction in the presence of vitamin C. The biuret reaction was a reaction of a protein under the action of a biuret reagent, which would produce a purple complex. However, the biuret reaction could not occur due to the lack of structural properties of the protein. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

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2026-07-01 17:44

Biuret reagent test protein reaction conclusion

When the biuret reagent was used to detect protein, when the base contained a peptidic bond (a peptidic bond), the copper in the test solution would coordinate with the peptidic bond, and the complex would turn purple. The color of the purple complex produced by the reaction was directly proportional to the concentration of the protein, and it had nothing to do with the molecular weight of the protein and the composition of the protein. The concentration could be analyzed by a prismatic method, and the wave-length in the ultraviolet-visible spectrum was 540mn. The sensitivity of the identification reaction was 5 - 160g/ml, and the identification reaction protein unit was 1 - 10g. Moreover, the compound molecules that could produce a purple reaction with the biuret reagent contained at least two peptidic bonds, so dipeptides could not be tested with it. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

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2026-07-06 07:54

The reaction between urine and biuret reagent before exercise

There was no protein in urine, so there was no purple reaction between urine and biuret reagent before exercise. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

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2026-07-01 09:41

What are the differences between qualitative and quantitative comic strips?

Qualitative comic strips focus more on the story, characters, and emotions. Quantitative ones might deal with data or measurable aspects like the number of panels or specific elements.

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2025-11-25 15:34

The reaction phenomenon of the reaction of nitrates and the reaction of the reaction of the nitrates and the sodiums

The reaction between the acid and the acid would result in the formation of a sulfuric acid deposit. The reaction equation was Na Chi SiOx +2HNOOx == H Chi SiOx +2NaNOOx, and the reaction phenomenon was the formation of a white deposit. In this reaction, the sulfuric acid did not show any oxidisation, but only showed its acidic nature. This was because the silicon in the silica was already in the highest state, and the acidic nature of the sulfuric acid was stronger than that of the sulfuric acid, which was in line with the principle of making a weak acid from a strong acid. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

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2026-08-07 12:39

Reaction equation phenomenon and explanation of the phenomenon of the reaction equation of the reaction of the reaction

There is no chemical reaction between NaCl2 and magnesiumcarbonate2. In chemistry, the conditions for metathesis reactions to occur were the formation of precipitations, gases, or water. After mixing the two, the conditions for the metathesis reaction were not met, so there was no reaction, no reaction equation, and no reaction phenomenon. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

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2026-07-15 14:10

What is the reaction of the aluminum thermal reaction? Is it an exhalation reaction?

Aluminiothermic reaction was a kind of oxide-reduction reaction between aluminum and metal or non-metal compounds at high temperatures. Aluminiothermic reaction was an exhaling reaction, and its heat release was very large, usually enough to heat the product above the melting point, and the reaction could generally occur locally and be self-sustaining. This characteristic also reflected the energy-saving characteristics of the reaction. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

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2026-07-02 12:12

What is the reaction of the rearrange reaction?

Rearrangement reaction refers to the migration of certain atoms or groups in the molecules of organic compounds under certain reaction conditions, resulting in the reorganization of the molecular structure. For example, the Beckmann Rearrangement Reaction was a reaction in which ketoximes were rearranged into N-substituted diamides under the action of an acidic catalyst; the Cope Rearrangement Reaction was a (3,3)-sigma-shift rearranging reaction of 1,5 -diene; the Hofmann-Martius Rearrangement Reaction was a reaction in which N-alkylated aromatic compounds were rearranged to the corresponding o-/p-alkylated aromatic compounds under the action of acid catalyst; the Curtius Rearrangement Reaction was a type of nuclopathic rearrangements. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

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2026-07-05 08:53

Reaction Formula of Substitution Reaction for the addition reaction of organic substances

Additional reaction: - The addition reaction of ethene and Bromine: <<CH2 = CH2 + Br2> - The addition reaction of ethene and hydrogen bromidate was as follows: <CH2 = CH2> - Under certain conditions, addition reactions could also occur between ethene, hydrogen, and water. Since the chemical properties of ethyne (carbon-carbon triple bond) were similar to that of alkene (carbon-carbon double bond), similar addition reactions could also occur. - The aromatic ring can undergo an addition reaction with hydrogen (in the presence of a catalyst such as Ni). - Aldol groups can undergo a reduction reaction (addition reaction), such as: <anno data-annotation-id ="00000000 - 4c00 - 4c00 - 8c00 - 9c00 - 9c000b000000"> CH3CH20H </anno>. Substitution reaction: - The substitution reaction between methane and Cl2: CH4 + Cl2. - The substitution reaction of the aromatic ring: For example, the substitution reaction with the aromatic group using FeBr3 as a catalyst; the nitration reaction with the aromatic group using concentrated sulfuric acid under heating conditions (the hydrogen on the aromatic ring is replaced by the nitrogen group). - The substitution reaction of the halated carbon was as follows: <<CH3CH2Br2>+<NaBr2>>. - The substitution reaction of alcohol: <CH3CH20H>+<br>> longrightarrow <CH3CH2br>+<H2O>> - The ester's cleavage reaction (which can be seen as a substitution reaction):<CH3COOCH2CH3 + H2O> <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

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2026-07-13 20:02
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