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A novel RT-qPCR based assay is a new and innovative method in molecular biology. It's developed through careful design of primers and probes, optimization of reaction conditions, and validation using various samples.

One possible novel method could involve using advanced enzymes and improved primers for more accurate and efficient amplification.

There could be a 'rt extra life rwby fanfic' that delves into the backstories of the other characters in RWBY. For example, it could show how Blake's past affects her decisions in a new situation within the fanfic's plot. This kind of story can add more depth to the existing RWBY universe and give readers a fresh perspective on the characters they already know and love.

The story of Xi Men Qing and Pan Jinlian was a classic plot in the Chinese classic novel Water Margins. Pan Jinlian was the daughter of a county magistrate's family, while Ximen Qing was a rich man. The two of them met and fell in love. However, due to Ximen Qing's power and wealth, he was envied and ostracized by others. In the end, he divorced his wife Wang Liu 'er and married Pan Jinlian. However, Pan Jinlian was not as happy as she looked. When she had committed adultery with Xi Men Qing, she had also seduced other men, which made Xi Men Qing feel extremely dissatisfied and guilty. The relationship between the two gradually deteriorated. In the end, Ximen Qing lost his footing and fell off the cliff after drinking, and Pan Jinlian committed suicide because of her excessive grief. In this story, the relationship between Ximen Qing and Pan Jinlian was full of deception, betrayal, and desire, becoming a classic villain in the novel.

Yes, JJK has finished in manga. The story has reached its conclusion.

It's possible that the JJK manga is ending. Maybe the story has reached a natural conclusion or the creator has decided to wrap it up.

Yes, it's great! The plot is engaging and the art style is amazing.

Yes, it is. The final chapters have been released.

Yes, it might be on break. Sometimes creators need a break for various reasons like planning or recharging.

The JJK manga is over. It had a defined ending that wrapped up the plotlines and characters' arcs.