Microscope lens modification photography
There were many ways to modify a microscope lens for photography. For example, a microscope lens could be modified into a camera lens for shooting, but this modification needed further improvement. There were also people who used the Hikvision camera and microscope lens to modify the electron microscope, but they might encounter some problems during the modification process. For example, the image was unclear, which might be a problem with the camera. When modifying, they had to consider the compatibility of the lens and the equipment. For example, when combining the microscope lens with the Hikvision network camera, they had to solve problems such as sensors, equipment errors, video storage, and so on. It may require the assistance of software such as the computer video recording software, the IVS- 4200. Read more exciting novels for free
Microscope lens opens up a new world of photography
The microscope lens brought new possibilities to photography. On the one hand, there were microscope lenses specially designed for mobile phones. For example, the microscope lens developed by the University of Science and Technology of China could be attached to mobile phones. It could support up to 400 times magnification. It could make the object captured by the mobile phone reach the level of an entry-level microscope, and its resolution could be adjusted to 2 millimeters. There were also 100x magnifying microscopes with LED lights from the Apexel brand that were suitable for most smart phones. They could be used for jewelry, insects, jade identification and exploration. On the other hand, traditional microscopes also had their own characteristics in terms of functions and application scenarios. For example, a continuous zooming microscope was suitable for maintenance scenarios. The adjustment range of the objective lens was generally between 0.65X and 4.5X. During maintenance, a 10X objective lens was generally used. If it was specially used to repair fingerprints and flying lines, a 20X eyepieces might be needed. The eyepieces also involved parameters such as the size of the field of view and the high viewpoint. In addition, there were also corresponding considerations in the selection of the light path, the magnifying lens, the light source, and the microscope stand. For example, the light path should be transparent without dizziness, the magnifying lens should be matched according to the needs, the appropriate light source should be used (such as a halo light source is recommended for long-term use), and the swing arm stand should be used for convenient movement. Whether it was for exploring the microscopic world, scientific research, identifying objects, or using them in special situations, these microlenses had opened up a new world for photography. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Microscope objective lens parameters
The parameters of the microscope objective lens mainly included the following aspects: 1. ** Magnification **: It can be divided into low-power objectives (such as 2x, 4x/5x, and 10x), medium-power objectives (such as 20x, 40x), and high-power objectives (such as 60x/63x, 100x). Different magnifying lenses used different media. High power lenses (60x and 100x) often used oil to obtain high resolution, while lenses with lower magnifying powers used air as the medium. 2. ** Observation method **: There are many types of microscope objectives. The common observation methods marked on the lens shell are: FF (bright-field), TF (dark-field), PL (phase contrast), Po (polar light), DIC (differential interference), FF (fluorescence observation, such as blue, green, purple, etc.), UVFO (ultraviolet-fluorescence observation), etc. 3. ** Chromatic Deviation correction ability **: - Achromatic objective lens: It can only correct the color difference of red and blue light. - Semiapochromatic objective (TL): Able to correct the color difference of red, green, and blue light. - Apoachromatic objective lens (Apo): It can correct the color difference of red, green and blue light twice, and can correct the spherical aberrations of red and blue light at the same time. 4. ** Flat-field correction function **: Choose an objective lens with flat-field correction function (marked with Plan) for better imaging effect. For example, a flat-field achromatic objective (Ach) could be used to view transparent sections; a semi-apochromatic objective (TL) could be used to view fluorescence, which had a long working distance and could be used to observe glass slips and petri dishes; a full-apochromatic objective (Apo) could be used for better imaging effects. However, Apo objectives did not have a long working distance and were only suitable for viewing glass slips, not for viewing thick samples such as petri dishes or culture bottles. 5. ** Numeric Aperture (NAC value)**: This is the main parameters of the objective lens and the condensing device. It is directly proportional to the resolution of the microscope, directly proportional to the magnifying power, and inverse proportional to the depth of field. UA = n * sin alpha. Under the same amplification, the higher the UA, the better. In the case where the working distance is satisfied, the objective lens with a high UA value should be selected. The numerical aperture of the dry objective lens was 0.05 - 0.95, and the numerical aperture of the oil-immersed objective lens was 1.25. 6. ** Working Distance (WD)**: - Ordinary working distance objective lens: The working distance is small, and the slices can be observed, but the petri dish cannot be observed. - [Long working distance objective lens: Used for inverted microscope. It can be used for microscopic examination of tissues, suspension, and other materials.] 7. ** Glass slide thickness **: After the optical type of the objective lens (infinity/0)(210/0), the number behind the slash represents the applicable glass slide thickness. For example,(infinity/0.17)(210/0.17), the applicable glass slide thickness is 0.17 mm. The objective lens with a higher NAC value was more sensitive to the thickness of the cover glass. Some objective lenses with high NAC values and those with long working distances had an adjustment ring for the thickness of the cover glass, which could be used for observation in petri dishes. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
First-ever microscope lens
The Oppo Find X3 series launched a cell phone with a microscope lens. The microscope lens had a 60x magnifying power, and the rear was equipped with a 1 billion color dual main camera (composed of a 50-million-pel imx766 free-form lens, a 50-million-pel imx766 OIS optical image stabilization lens, a 13-million-pel telephoto lens with a maximum 20x digital zoom, and a microscope with a 60x magnifying power). In addition, the Realme GT2 Pro also launched the microscope 2.0 lens, which increased the object distance by nearly two times. It also supported the skin texture and skin color detection function, and upgraded the depth of field by four times. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Microscope single-lens reflex interface
The reference mentioned that some microscopes were equipped with a single-lens reflex mount. For example, the Phoenix XSP - 36 biological microscope had a single-lens reflex mount, the Phoenix RH50 - 3A43L-PL biological microscope had a single-lens reflex mount, and the Saga Binocular Professional Microscope 1600X could be connected to a single-lens reflex mount (similar to the function of a single-lens reflex mount). However, there was no detailed explanation of the single-lens reflex mount. I'm sorry, but I don't know how to answer further based on the information I found. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
How to adjust the position of the microscope lens
For a common microscope, the steps to adjust the lens position (focusing) were as follows: First, place the sample on the stage and fix it, then turn the coarse focusing wheel to move the distance between the objective lens and the sample. At first, the objective lens was quickly moved down to the lowest position, and then slowly raised by turning the coarse focus wheel until a blurry but visible image appeared in the field of view, indicating that the correct focus point was approaching. After that, the fine focus wheel was rotated slightly to further improve the image clarity, and the fine adjustment was made until the desired clarity was achieved. If you need a higher magnification, choose the appropriate objective lens and repeat the above steps to obtain a better focusing effect. During use, you should also ensure that the lighting is appropriate according to the actual situation, such as adjusting the brightness controller. Some models may need to turn on or turn off additional light sources. In addition, before the operation, you need to turn the changer so that the low-power lens is aimed at the light hole, and the larger aperture is aimed at the light hole. One eye is focused on the eyepieces, and the other eye is open. Turn the mirror so that the light is reflected into the lens barrel through the light hole. After seeing the bright circular field of view, you can adjust the position of the lens. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
How to adjust the brightness of the visual microscope lens
The adjustment of the brightness of the field of view under the visual microscope lens was mainly related to the mirror and the shutter. The aperture and the mirror were used together to ensure the best light required. The adjustment steps were as follows: Move the revolver with your thumb and middle finger (do not move the objective lens with your hand), so that the low-power lens was aimed at the light hole of the mirror table (when you hear the knocking sound, it means that the optical axis of the objective lens has been aligned with the center of the tube). Open the aperture, raise the light collector, and turn the mirror to the light source. Observe with the left eye on the eyepieces (open the right eye). At the same time, adjust the direction of the mirror until the light in the field of vision is evenly bright. The size of the aperture could also be changed by adjusting the shutter. When the field of vision was bright, a large aperture was used, and when the field of vision was dark, a small aperture was used. As for the mirror, a concave mirror was used when it was bright, and a flat mirror was used when it was dark. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Scanning electron microscope photography malfunction
Scanning electron microscope photography may have a variety of faults. The following are some common faults and related analysis: - ** Poor tube vacuum **: When the tube vacuum is poor, the 30kV-high voltage will be added to the grid cap for a few minutes, and the tube will be seriously polluted. The tube vacuum will directly affect the normal operation of the scanning electron microscope. Because the tube has a high vacuum, the 5 - 30 volts high voltage can work under good conditions, so the high-brightness electron gun can obtain a thinner electron probe. According to the working principle of the vacuum system, the flow chart of high and low vacuum fault analysis could be sorted out and drawn, and the part discrimination method could be used to check step by step, narrow the scope, and find the fault. - ** Weak image signal, small contrast **: When this kind of fault occurs, it may be caused by many factors. If the acceleration voltage is not selected properly, it is necessary to select a suitable acceleration voltage for samples with different conductivity, stability, and vulnerability. Otherwise, the observed image is not the true appearance of the sample. For samples with good electrical and thermal conductivity, such as metals, a higher voltage can be used for observation, such as 10 volts and above; for some samples with poor electrical conductivity but relatively stable, a medium acceleration voltage can be used, such as about 5 volts; for some samples that are easy to damage, such as high molecular materials, biological materials, etc., a lower voltage may be needed, such as 2 volts or below. - ** Interferential signal appears when observing the image **: Internal interference may be due to circuit self-oscillation, high-voltage ignition, large power ripple, etc. You can start from the three sets of low voltage of the vertical amplifier. If it is determined that the power supply itself has self-excited oscillation, it can be solved by adding a vibration elimination circuit between the two stages of the difference amplifier to reduce the output ripple to less than 15 millivolts. The sawtooth output wave is normal, thus eliminating the net interference on the screen. - ** Aspect problem **: Image dispersion affects image clarity, especially when taking high-resolution pictures (such as taking high-resolution pictures with high acceleration voltage and low beam current). The image would be stretched and deformed during the focusing process, losing its original shape. When the image of the scanning electron microscope showed some aberrations, the image would be stretched when it was focused. Antagonism required the experimenter to have a wealth of operational experience in order to accurately identify and eliminate the image. - ** Phenomenon of carbon deposit **: After observing an area for a period of time, there may be obvious carbon deposits on the surface of the sample. The surface of the particles seems to have a "thin film", which will affect the photo effect of the sample and reduce the clarity of the image. It will also affect the accuracy of the energy spectrum analysis of light elements. The main source of carbon deposits was the sample.(contact with grease and other impurities during preparation or storage), electron microscope components (for example, the vacuum system of the scanning electron microscope has an oil pump, and a trace of oil vapor enters the electron microscope chamber; improper operation may cause the sample to pollute the accessories, etc.), operators (contact with samples and accessories may cause pollution), electron microscope room environment (electron microscope and samples are exposed to a dusty environment for a long time, or powder sample preparation in the electron microscope room may cause powder floating pollution), etc. In response to these situations, corresponding measures could be taken, such as cleaning and drying the contaminated samples with alcohol and other organic liquids; for the vacuum system of the Feiner electron microscope, which was an oil-free mechanical pump, the user should properly keep the sample cup, store it in a sealed bag or a clean box when not in use, and wear gloves to avoid grease contamination during operation; the operator should wear gloves or use clean sample preparation tools to operate; do not perform powder sample preparation in the electron microscope room, etc. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Landscape photography lens
In landscape photography, different types of lenses had their own characteristics and uses. ** 1. Wide-angle lens ** 1. ** Specialties ** - It provides a wide field of vision and can include the entire landscape into the picture, from the front to the horizon. - With the widest depth of field, it can focus on the entire scenery, so that the front and back scenes of the subject can be clearly reproduced on the screen. - Distortion would magnify the objects in the background, showing close-up details, and emphasizing the guide lines, enhancing the dynamic of the composition. 2. ** Exemplary recommended shots ** - Nikon Z14 - 24mm F2.8S: An all-round upgrade. Its design and performance surpass the same type of lens with a single-lens reflex mount. It is no longer a big bulb design. Its size and weight are greatly reduced. It weighs only 650g. It comes with two disassembled hoods and a rear filter slot. It is excellent in glare, distortion, and dispersion control. - Canon RF15 - 35F2.8LIS USM: A super wide-angle lens with excellent image quality and optical performance. The wide-angle end is wider by 1mm, and the viewing angle is increased to 110°. It has a non-bulb structure design, 82 filter size, reduced cost, and a weight of 840g. It has good anti-glare performance. It is equipped with optical image stabilization to achieve 1/2 second hand-held shooting. - SIGMA16 - 28mm F2.8DG Continental: Picture quality comparable to Tamron 17 - 28mm F2.8, focal length 1mm wider than 17 - 28mm. - You could also consider a 16 - 35 wide-angle zoom lens. ** 2. Telephoto lens ** 1. ** Specialties ** - A narrow field of view, suitable for trimming excess elements and focusing on small but beautiful scenes, such as the curve of a mountain, the reflection of a pond in the distance, or the silhouette of a tree. - It could compress the space, making it easier to highlight the main body. It was easy to get started, and the composition was extremely flexible. It had the ability to make deductions. 2. ** Exemplary recommended shots ** - 70 - 200mm focal length: - Tenglong 70 - 200 (G2): Fast focusing speed, improved edge quality, brand new price 7490 yuan. - Sony users could choose the 70 - 180mm (A056) from Tenglong, priced at 6890 yuan. From the MTF curve, the sharpness of the 70mm end and 180mm end was close to the original 70 - 200GM. - Canon users with high cost-performance ratio could choose the White Rabbit EF70 - 200mm f/2.8L IS II USM (second generation), and the second-hand quality was more than 7000 yuan; those with weight requirements could choose the 70 - 200mm f/4L IS II USM; Canon non-reflective users could choose the lightweight RF70 - 200F4, and those who pursued higher directly chose the RF70 - 200F2.8. - Nikon users could choose to use the Electromagnetic Gun or the 70 - 200 Z-mount. - If the users didn't mind the focus and weight, the 70 - 200F2.8 first-generation model was very cost-effective. After the second-generation model was released, the first-generation model would have a small price reduction. The second-generation model was 29% lighter than the first-generation model and the difference in focus. - 100 - 400mm focal length: - The sub-factory's Sigma100 - 400 and Tenglong 100 - 400 could be selected. They were suitable for shooting birds when they were occasionally idle and lotus flowers during the lotus season. The Sigma100 - 400mm part of the sharpness for the Sony-compatible users was higher, but the focus was slow and the light was slightly weak. It could be used as a tourist attraction. The Nikon Z100 - 400mm F4.5 - 5.6 VR S had fast focusing speed and no shortcomings. It was the top lens of its type. The quality of the lens was good from the center to the edge. The anti-glare, dispersion control, and dark angle control were in place. It was suitable for shooting sunrise and sunset in landscape photography. It also had twice the effect with half the effort in broad scenes such as mountains and grasslands. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Reflex lens photography
The works shot with a reflective lens had a unique effect. For example, the wild cypress flowers in Northeast China shot by Nikon Z8 with NiSi 250mm F5.6 ReflexTF lens, as well as the peony flowers under the backlighting shot by this lens, presented a hazy, dreamy and gentle effect; peony flowers and barnyard grass could be covered with dewdrops, and the roadside flowers under the backlighting with the morning sun sprinkled on them were particularly dazzling; The picture of the path in the pear tree garden was taken through this lens with five multiple exposure shots. The picture was soft and transparent, with comfortable retro warm colors. It looked calm, clean, and more like an oil painting atmosphere. These works could display the unique haziness effect of the lens. When the point light source in the background or when there were dewdrops on the grass or ripples on the river under the backlighting, the highlights would form a charming ring-shaped defocused " donut ". The more highlights outside the depth of field, the brighter the donut effect would be. At the same time, it could also show the retro and unrestrained two-line effect. Its tone and color were unique, giving it a sense of oil painting and presenting an artistic two-line touch. The soft, retro oil painting's tones and shades gave off a dreamy effect. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Private photography lens
Generally, when taking private photos indoors, many photographers would choose 28mm and 35mm fixed-focus lenses for shooting because the distortion of the ultra-wide-angle lens was too serious. Among them, 28mm f1.4 was known as the best lens for shooting private photos. Its focal length was wide enough to achieve the blurring effect. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>