This question was difficult to answer because there were many poor male and female protagonists in anime. Everyone might have different opinions. However, here are some of the most beloved and most pitiful anime male and female protagonists: 1. Your Name. Makoto Shinkai and Akari Shinohara: They were lovers who liked each other, but they had to leave each other in the face of disasters such as earthquakes and tsunamis, and they could not be together in the end. 2. Alan and Yumir in The Attack of the Giants: They were a pair of brothers. Alan's sister was eaten by a giant, and Yumir sacrificed his life to protect Alan. 3. Tanjiro and Wuxian in " The Blade of Ghost Destruction ": They were fellow disciples. Tanjiro embarked on a journey to find a ghost in order to save his sister, while Wuxian was killed by his sister. 4. Okabe Rentaro and Rena in The Gate of the Stone of Destiny: They were a couple who liked each other, but because of their research, they needed to conduct experiments that eventually led to their deaths. 5. Kimki Yan and Reina in " Tokyo Ghoul ": They were the companions of Kimki Yan, who became a ghoul in order to save his sister, and Reina, who was infected by the virus and became a ghoul. These were just some of the most pitiful male and female protagonists who were loved by the audience. There were also many other cartoon characters who had pitiful stories.