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Biology elective, a must-memorize knowledge point encyclopedia, PEP edition

2026-09-28 17:09
2026-09-28 21:47

The following are some of the must-memorize knowledge points for the Biology elective 1 of the People's Education Version: 1. **DNA related knowledge ** - ** Dissolution **: DNA and protein have different dissolutes in different concentration of NaCl-based solutions. This feature can be used to separate DNA from impurities. DNA is not dissolved in alcohol solution, but some protein is dissolved in alcohol, which can further separate DNA and protein. - ** Resistance to acids, high temperatures, and deterrents **: The protein has no effect on DNA. Most protein cannot withstand high temperatures of 60 - 80°C, while DNA will only denature above 80°C. The deterrents can disintegrate the cell membrane, but it does not affect DNA. - ** Identification **: Under the conditions of boiling water bath, DNA will be dyed blue when it comes into contact with diamines. diamines can be used as a reagent for DNA identification. - **DNA extraction and identification of experimental materials **: Biological materials containing DNA are acceptable, but biological tissues with relatively high DNA content are better. - ** Disrupting cells to obtain the Dna-containing fluid (chicken blood cells and plant cells as examples)** - Chicken blood cells: Distilled water was added to the chicken blood cell fluid, stirred with a glass rod, and filtered to obtain the filtered fluid. Distilled water was a hypotonic liquid for chicken blood cells. A large amount of water entered the blood cells and caused them to burst. Stirring accelerated the rupture and released DNA. - Plant cells (taking onions as an example): Add the washing agent and salt to the chopped onions, stir and grind them thoroughly, and then filter them to obtain the grinding liquid. The washing agent dissolved the cell membrane, which was conducive to the release of DNA, while salt (NaCl2) was conducive to the dissolution of DNA. If the grinding was insufficient, the DNA in the nucleus would not be completely released, which would affect the results of the experiment. The filtered liquid of this step may contain impurities such as protein, glycan, and R A. - ** Removing impurities in the filtered solution **: There are three methods: one is to use the different dissolutes of DNA in different concentration of NaCl-solution; the second is to use a protein to decompose the protein but not the DNA; the third is to use the different denature temperatures of the protein and DNA. - **DNA extraction and identification procedures (onion as an example)** - Weigh 30 grams of chopped onions, add a small amount of Quartz sand to aid grinding, pour 10mL of 2-mole/L solution of NaCl3 and grind them thoroughly (onions can be treated first to reduce irritation). The purpose of grinding is to break the cells and let the DNA dissolve in the NaCl3 solution. There is no need to grind them into a porridge paste, and you can't use a whisk. - After grinding, it was filtered with a funnel and gauze to obtain the filtered liquid. - Add 20ml of 95% alcohol solution to the filtered solution (pour it slowly along the beaker wall without shaking or stirring). The upper layer of the solution will separate out a white, fibre-like viscous substance (DNA), which can be gently rolled up with a glass rod. 2. ** Traditional fermentation technology ** - ** Fruit wine making ** - ** Principle **: Yeast's anoxide-free breathing, the reaction formula is C6H12O6 → 2C2H50H +2CO2 + energy. - ** Source **: Wild yeast attached to grape skin or artificially cultivated yeast. - ** Condition **: 18 - 25 ° C, sealed, venting (CO2) at intervals. - ** Detection **: Under acidic conditions, the reaction between the alcohol and the potassium bichromate is grayish-green. - ** Fruit vinegar production ** - [Principle: Aerobic Breathing of Acetic Acid bacteria.] When O2 and sugar source were sufficient, sugar would be decomposed into vinegar; when O2 was sufficient and sugar source was insufficient, alcohol would be transformed into jumped and then into vinegar. Read more exciting novels for free

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2026-10-06 15:08

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2026-10-06 15:07

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2026-10-06 15:07

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2026-10-06 15:07

Primary school students 'anti-epidemic record form template

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2026-10-06 15:06

Joe dressed up as a mother of five

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2026-10-06 15:06

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2026-10-06 15:05

What is the name of the female protagonist of the Tang Dynasty novel, Princess Jin Yang?

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2026-10-06 15:05
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