Gary could be the main antagonist. He has always been Ash's rival and seeing Ash turn evil, he might feel responsible to stop him. Gary could gather his own team of powerful Pokémon and use his wits to oppose Ash at every turn.
One possible plotline could be that Ash is betrayed by his friends. This heartbreak and sense of abandonment turns him evil. He then starts using his Pokémon in cruel ways to seek revenge on those who wronged him.
One popular 'ash becomes evil after betrayal fanfiction' is 'Ash's Dark Descent' which explores how Ash's trust is shattered and he turns to the dark side. It has some really intense scenes where you can see his transformation.
One possible scenario in this fanfiction could be that Ash has to overcome his toughest opponents yet in Unova. He might face trainers with unique and powerful Pokémon that he has never encountered before. His Pikachu would surely play a crucial role, using its speed and electric attacks to outwit opponents.
One idea could be that Ash has a strange encounter in a mysterious forest. There, he finds a Riolu in distress. As he tries to help the Riolu, a strange energy transfer occurs, and he starts to take on some of Riolu's characteristics. He might gain Riolu's heightened senses, like its ability to sense auras more acutely. This could lead to new adventures as he has to adapt to these changes while still trying to pursue his Pokémon journey goals.
In many fanfictions, Ash achieving the status of a Pokemon Master often involves him having extraordinary battles. He might defeat powerful legendary Pokemon that no one has ever tamed before. His Pikachu could evolve into a more powerful form, which gives him an edge in battles. Also, his bond with all his Pokemon, not just Pikachu, becomes so strong that they can execute complex strategies without any communication barriers.
I'm not entirely sure specifically about this 'evil ash betrayed fanfiction' as there could be many different interpretations. It might involve the character Evil Ash in a situation where he betrays someone or something within the context of the fan - created story.
Latios would obviously be a key character. Also, Pikachu, Ash's loyal Pokémon partner, would be there. Maybe Misty or Brock could also be part of the story, with them either supporting or being skeptical of Ash's newfound admiration for Latios.
His Pokemon could be his potential allies. They've been with him through thick and thin and would likely still support him even with his new vampire state. As for enemies, there might be those in the Pokemon world who fear or dislike vampires and would see Ash as a threat. Maybe some powerful and evil Pokemon trainers who want to use his new situation against him.