In certain comic continuities, Gwen Stacy is alive. However, her status has changed throughout different comic arcs and reboots. Sometimes she's been brought back through various means in the fictional comic world.
Gwen Stacy is not trans in the comics. The character has been presented in a conventional gender role throughout the comic series. There have been no storylines suggesting otherwise.
Gwen Stacy was an important figure in the Spider-Man comic universe. She was smart, kind, and had a complex relationship with Peter Parker. Her death had a huge impact on the story.
Gwen Stacy's story takes a dark turn in the comics. She becomes a victim of circumstances involving a supervillain, and her death leaves a lasting mark on the comic book narrative.