Absorbency of blank value of nitrogen and nitrogenIn the detection of nitrogen and nitrogen, the blank light absorption referred to the light absorption obtained by measuring the light source, the cuvette, and other optical systems without any sample. The blank absorption range of nitrogen nitrogen was a key factor in the process of nitrogen nitrogen detection. It played an important role in ensuring the accuracy and reliability of the test results. The reagent blank for the determination of nitrogen and nitrogen shall have an absorption value of not more than 0.030 (10mm cuvette), and the test water blank shall have an absorption value of not more than 0.030 to meet the requirements of determining the absence of nitrogen and nitrogen.
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Nessler's reagent standard curve for determining nitrogen nitrogenThe following is the content related to the standard curve of Nessler's reagent for determining nitrogen and nitrogen:
1. ** Steps of Drawing the Calibrating Curv **
- Add 0.00, 0.50, 1.00, 2.00, 4.00, 6.00, 8.00, and 10.00ml of the standard working solution of nitrogen into eight 50ml color comparison tubes, respectively. The corresponding contents of the standard working solution of nitrogen and nitrogen are 0.0, 5.0, 10.0, 20.0, 40.0, 60.0, 80.0, and 100.0 ug, respectively. Add water to the marking line.
- Add 1.0ml of the solution of potassium-Na tartrate and shake it well. Then add 1.5ml of mercuric chloride-potassium iodide-potassiumoh solution or 1.0ml of mercuric iodide-potassium iodide-potassiumoh solution (Nessler's reagent) and shake it well.
- After standing for 10 minutes, measure the absorption with a 20-mm light path cuvette at a wave length of 420 mm and water as a reference.
- The absorption after blank correction was used as the ordinate and the corresponding nitrogen content (ug) was used as the abyssal coordinate to draw the correction curve. A 10mm cuvette can also be used according to the mass concentration of the sample to be tested.
2. ** Exemplary Data **
- For example, some data showed that when the nitrogen content was 0.000g, the light absorption was 0.028, and when the nitrogen content was 0.005g, the light absorption was 0.055. A standard curve was drawn based on these data points (the content of nitrogen and its corresponding light absorption). For example, the data was as follows:
- No. 1: Volume: 0ml, standard concentration of nitrogen nitrogen: 10 ug/ml (mass: 0 ug), absorption value: 0.096.
- No. 2: Volume: 1ml, standard concentration of nitrogen nitrogen: 10 ug/ml (mass: 10 ug), light absorption value: 0.14.
- Number 3: The volume is 2ml, the standard concentration of nitrogen is 10 ug/ml (mass is 20 ug), and the absorption value is 0.179. According to these data, a standard curve of the relationship between the nitrogen content and the light absorption can be drawn. This curve is generally linear, for example, it meets the linear equation of <y = 3.512x+ 0.0048>(where <y> is the corrected light absorption, and <x> is the nitrogen content), which is used to calculate the nitrogen content according to the measured light absorption.
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What will happen to the reaction of iron sulfuric acid and nitrogen nitrogen?Based on context alone
Iron Sulphate (Fe2 (SO)) and nitrogen nitrogen (usually in the form of NHin solution) will react.
In the solution, iron ions (Fe3) will be produced by the dissolution of iron sulfuric acid, and the iron ions will undergo a double decomposition reaction with the lithium ions (NH3). Iron ions were decomposed to produce iron (III), while the nitrogen ions were decomposed to produce nitrogen (III). The nitrogen ions were unstable and would decompose to produce nitrogen (III). The reaction equation was roughly as follows:
Fe₂(SO₄)₃ + 6NH₄⁺ + 6H₂O = 2Fe(OH)₃↓+ 3(NH₄)₂SO₄ + 6H⁺
(NH The reaction will continue to proceed to the right, and the nitrogen ions will be further decomposed to produce hydrogen gas that escapes from the system.
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There is color in the blank when the Nessler reagent is used to measure the nitrogen nitrogen.There may be the following reasons for the color of the blank in the Nessler's reagent:
1. ** The characteristics of the Nessler's reagent itself **: The Nessler's reagent is a slightly yellowish green transparent solution at room temperature. As the exposure time increases, it will gradually form a yellowish brown deposit, and the solution will gradually turn yellow. If the exposure time of the Nessler's reagent in the blank experiment was too long, it might cause color to appear.
2. ** Experiment operation factors **
- ** Cuvette problem **: If a 1cm cuvette is used, the blank value of the blank absorption is too high and is greater than 0.030, which may cause color to be seen. The first reason was the purity of the reagent, such as the reagent used containing an aminium salt (such as potassium-Na tartarate); the second reason was that the test water was contaminated and the introduction of an aminium salt.
- ** Inappropriate use of Nessler's reagent **: Nessler's reagent needs to be kept at room temperature before use, and it cannot be shaken before use. It should be used by absorbing the supernate. If this operation is not followed, the blank color may be affected.
3. ** Purity and contamination of reagents **
- [** Impurity in the reagent **: The order of adding the medicine in the preparation of the Nessler's reagent is different, and the phenomenon of different colors often occurs.]
- ** Contained **: If the experimental water or other reagents are contaminated with either nitrogen or nitrogen, the Nessler's reagent will also produce a color reaction in the blank experiment.
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How to test for nitrogen?The test method for the colorless and pungent odor of the gas was as follows: First, it could be tested with a wet red litmus test paper. If the test paper turned blue, it proved the existence of the gas. Second, a glass rod dipped in concentrated sulfuric acid was used to approach the gas to be tested. If white smoke was produced, it indicated the existence of the gas.
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What is the nitrogen origin story?Nitrogen's origin dates back to the early universe. In the extremely high - energy environment of the young universe, elementary particles combined and formed atoms. Nitrogen atoms were created as part of this process. Over time, in the cores of massive stars, more nitrogen was synthesized through fusion reactions. When these stars exploded as supernovae, they scattered nitrogen and other elements across the cosmos, enriching the matter that would eventually form our solar system and Earth.
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2024-12-12 18:55
A reagent for absorbing nitrogen in the laboratoryThe reagents used in the laboratory to absorb nitrogen included saturated salt water (saturated NaCl-solution), acidic solutions such as calcium chloride-acid, sulfuric acid, or sulfuric acid, soda lime, quicklime, silica gel, and a white to light gray powdered solid (composed of a mixture of organic salt, metal dioxide, and surface modifying agent). Among them, saturated salt water absorbs the physical property of the water that is easily dissolved in the water, calcium chlorideabsorbs the chemical property of the octa-ammine calcium chlorideby reacting with the hydrogen gas, acidic solution absorbs the hydrogen gas by reacting with the basic hydrogen gas to produce salt and water, soda lime, quicklime, and silica gel can be used as the basic drying agent to absorb the hydrogen gas, and the hydrogen absorbing agent can absorb the nitrogen and hydrogen gas due to its multi-pore structure and suitable hole diameter.
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2024-11-14 22:42