No other names were found for the Nessler reagent. Its English name was Nessler. "Little Fox Fairy" is equally exciting. Everyone is welcome to click and read it!
Nessler's reagent contained mercury and other components. Mercury was toxic, and if it came into contact with the skin, it needed to be washed in time. Wearing gloves could prevent the skin from coming into direct contact with the Nessler's reagent and prevent the harmful substances in the reagent from causing damage to the skin, so wearing gloves was useful. "Little Fox Fairy" is equally exciting. Everyone is welcome to click and read it!
When Nessler's reagent was used for the determination of nitrogen and nitrogen, the suitable range of color should be controlled between 11.8 - 13.4. When the pH-value was lower than 11.8, there would be no color reaction; when the pH-value was higher than 13.4, the solution would immediately become turbid, and the absorption could not be measured. However, some studies have shown that the optimal color development is at a pH11.8 - 12.4. "Little Fox Fairy" is equally exciting. Everyone is welcome to click and read it!
There were various prices for Nessler's reagent. For example, a 100ml bottle of Nessler's reagent cost 118.50 yuan, a 500ml bottle of Nessler's reagent cost 64.6 yuan, and some cost 9 yuan. "Little Fox Fairy" is equally exciting. Everyone is welcome to click and read it!
The following is the content related to the standard curve of Nessler's reagent for determining nitrogen and nitrogen: 1. ** Steps of Drawing the Calibrating Curv ** - Add 0.00, 0.50, 1.00, 2.00, 4.00, 6.00, 8.00, and 10.00ml of the standard working solution of nitrogen into eight 50ml color comparison tubes, respectively. The corresponding contents of the standard working solution of nitrogen and nitrogen are 0.0, 5.0, 10.0, 20.0, 40.0, 60.0, 80.0, and 100.0 ug, respectively. Add water to the marking line. - Add 1.0ml of the solution of potassium-Na tartrate and shake it well. Then add 1.5ml of mercuric chloride-potassium iodide-potassiumoh solution or 1.0ml of mercuric iodide-potassium iodide-potassiumoh solution (Nessler's reagent) and shake it well. - After standing for 10 minutes, measure the absorption with a 20-mm light path cuvette at a wave length of 420 mm and water as a reference. - The absorption after blank correction was used as the ordinate and the corresponding nitrogen content (ug) was used as the abyssal coordinate to draw the correction curve. A 10mm cuvette can also be used according to the mass concentration of the sample to be tested. 2. ** Exemplary Data ** - For example, some data showed that when the nitrogen content was 0.000g, the light absorption was 0.028, and when the nitrogen content was 0.005g, the light absorption was 0.055. A standard curve was drawn based on these data points (the content of nitrogen and its corresponding light absorption). For example, the data was as follows: - No. 1: Volume: 0ml, standard concentration of nitrogen nitrogen: 10 ug/ml (mass: 0 ug), absorption value: 0.096. - No. 2: Volume: 1ml, standard concentration of nitrogen nitrogen: 10 ug/ml (mass: 10 ug), light absorption value: 0.14. - Number 3: The volume is 2ml, the standard concentration of nitrogen is 10 ug/ml (mass is 20 ug), and the absorption value is 0.179. According to these data, a standard curve of the relationship between the nitrogen content and the light absorption can be drawn. This curve is generally linear, for example, it meets the linear equation of <y = 3.512x+ 0.0048>(where <y> is the corrected light absorption, and <x> is the nitrogen content), which is used to calculate the nitrogen content according to the measured light absorption. "Little Fox Fairy" is equally exciting. Everyone is welcome to click and read it!
There may be the following reasons for the color of the blank in the Nessler's reagent: 1. ** The characteristics of the Nessler's reagent itself **: The Nessler's reagent is a slightly yellowish green transparent solution at room temperature. As the exposure time increases, it will gradually form a yellowish brown deposit, and the solution will gradually turn yellow. If the exposure time of the Nessler's reagent in the blank experiment was too long, it might cause color to appear. 2. ** Experiment operation factors ** - ** Cuvette problem **: If a 1cm cuvette is used, the blank value of the blank absorption is too high and is greater than 0.030, which may cause color to be seen. The first reason was the purity of the reagent, such as the reagent used containing an aminium salt (such as potassium-Na tartarate); the second reason was that the test water was contaminated and the introduction of an aminium salt. - ** Inappropriate use of Nessler's reagent **: Nessler's reagent needs to be kept at room temperature before use, and it cannot be shaken before use. It should be used by absorbing the supernate. If this operation is not followed, the blank color may be affected. 3. ** Purity and contamination of reagents ** - [** Impurity in the reagent **: The order of adding the medicine in the preparation of the Nessler's reagent is different, and the phenomenon of different colors often occurs.] - ** Contained **: If the experimental water or other reagents are contaminated with either nitrogen or nitrogen, the Nessler's reagent will also produce a color reaction in the blank experiment. "Little Fox Fairy" is equally exciting. Everyone is welcome to click and read it!
The reagents used in the laboratory to absorb nitrogen included saturated salt water (saturated NaCl-solution), acidic solutions such as calcium chloride-acid, sulfuric acid, or sulfuric acid, soda lime, quicklime, silica gel, and a white to light gray powdered solid (composed of a mixture of organic salt, metal dioxide, and surface modifying agent). Among them, saturated salt water absorbs the physical property of the water that is easily dissolved in the water, calcium chlorideabsorbs the chemical property of the octa-ammine calcium chlorideby reacting with the hydrogen gas, acidic solution absorbs the hydrogen gas by reacting with the basic hydrogen gas to produce salt and water, soda lime, quicklime, and silica gel can be used as the basic drying agent to absorb the hydrogen gas, and the hydrogen absorbing agent can absorb the nitrogen and hydrogen gas due to its multi-pore structure and suitable hole diameter. While waiting for the TV series, you can also click on the link below to read the classic original work of "Dafeng Nightwatchman"!
The reaction between the ester of a phenate and the Grignard reagent. In the university's organic chemistry experiment, there was a project to prepare trimethyol by reacting the Grignard reagent with the ester of a phenate. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>
Fehling's solution was Fehling's reagent. It was invented by the German pharmacist Herman van Fehling in 1849. It was often used to identify the existence of a reducing sugar. It could react with the reducing sugar (aldose) in the monolith to form a brick-red deposit.
The reagent blank is that, except for the sample solution, all the other reagents and solutions are added according to the conventional method. That is, in the process of chemical analysis, when only the color reagent has color and has significant absorption of light at a specific wave length, and other components have no obvious absorption or absorption, a blank solution without the substance to be tested (that is, no sample is added) can be used as a reference. This blank solution is prepared under the same conditions as the color reaction. Therefore, no sample was added to the reagent blank. "Little Fox Fairy" is equally exciting. Everyone is welcome to click and read it!
There was no protein in urine, so there was no purple reaction between urine and biuret reagent before exercise. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>