In Naruto, even though Tsunade was unmarried, the continuation and development of the Thousand-Hand Clan did not completely depend on Tsunade's marital status. The Thousand-Hand Clan had a deep foundation in the village and many ninja clan members. They played an important role in the construction of the village and the storage of ninja power. The other members of the family could still continue the family bloodline through normal reproduction. Moreover, the concept of family in the ninja world was also changing with the development of the times. It emphasized the inheritance of the ninja's ability, family honor, and many other aspects. It was not just to ensure the continuation of the family in the form of marriage.
The members of the Thousand Hand Clan included Thousand Hand Buddha, the Clan Leader of the Thousand Hand Clan during the Chaotic Era, and the father of the four brothers, Thousand Hand Pillar, Thousand Hand Fei, Thousand Hand Tile, and Thousand Hand Tile. Thousand Hand Pillar was the first generation Huoying, Thousand Hand Fei was the second generation Huoying, Thousand Hand Tile was the younger brother of the first generation Huoying Thousand Hand Pillar and Thousand Hand Fei, Thousand Hand Tile was the older brother of Thousand Hand Tile, and Thousand Hand Buddha was the third son of Thousand Hand Tile. Okami was the granddaughter of Pilama, a famous figure in the ninja world. She was one of the " Legendary Three Ninjas." After the death of the third-generation Hokage, she inherited the position of Hokage and became the fifth-generation Hokage.
The descendants of the Thousand Hand Clan were Thousand Hand Pillar, Thousand Hand Door, Thousand Hand Tile, and Thousand Hand Palm. The granddaughter of Thousand Hand Pillar was also a descendant of the Thousand Hand Clan. However, after the first and second generation, only Hokage, the fifth generation of the Thousand Hands Clan, appeared. The rest of the clansmen rarely appeared.
The members of the Thousand Hands Clan were Thousand Hands Buddha, the leader of the Thousand Hands Clan during the Age of Turmoil; the four brothers Thousand Hands Pillar, Thousand Hands Door, Thousand Hands Tile, and Thousand Hands Tile; Thousand Hands Pillar's granddaughter Tsunade; and the young ninja Thousand Hands Tile, who was killed during the Age of Turmoil.
Yes, Tsunei was from the Thousand Hands Clan. She was the granddaughter of the first generation Naruto Chishigama and his wife, Mizudo Uzumaki. She was a descendant of the Chishigama clan.
In the works related to Naruto, there was no clear mention of the exact time when the Thousand-Hand Clan disappeared. However, it was known that as time passed, the Thousand Hands Clan gradually declined. After the active period of the Thousand Hand Pillar and the Thousand Hand Door, the influence of the Thousand Hand Clan and the appearance of the clan members gradually decreased. For example, the granddaughter of the Thousand Hand Pillar became a relatively well-known figure in the later stages of the Thousand Hand Clan. After that, there was little mention of the overall size and activity of the Thousand Hand Clan. From the development of the plot, it was speculated that it might slowly integrate or decline in the process of the continuous development of the wooden leaf village, but there was no clear indication of the time when it disappeared.
Within the Thousand Hand Clan, the Thousand Hand Pillar was considered the strongest. As the reincarnation of Asura, the second son of the Six Paths Immortal, Zhu Jian had the complete bloodline of the immortal body. He had a powerful physique that far surpassed ordinary people and developed the secret technique of wood escape. During the Warring States Period, he led the Thousand-Hand Clan to fight against the Uchiha Clan. In the end, he persuaded the Uchiha Clan's leader, Uchiha Haberan, to reach an agreement to stop the war. They were both hired by the Country of Fire and established the Koba Hidden Village. Under his leadership, the Thousand-Hand Clan had great influence at that time.
The members of the Thousand Hand Clan were as follows: Thousand Hand Buddha, he was the clan leader of the Thousand Hand Clan and also the father of the four brothers, Thousand Hand Pillar, Thousand Hand Door, Thousand Hand Tile, and Thousand Hand Palm. Thousand Hand Pillar was the first generation Huoying; Thousand Hand Door was the second generation Huoying; Thousand Hand Tile was the younger brother of the first and second generation Huoying, the older brother of Thousand Hand Palm, and the third son of Thousand Hand Buddha. The Thousand Hands Clan was the descendant of Asura, the second son of the Six Paths Immortal. They were distant relatives of the Whirlpool Clan. Their clansmen were huge chakras and had strong vitality. They inherited the immortal body and were known as the Thousand Hands of the Forest. Since the Muye Village was established in the end, the symbol of the Thousand-Hand Clan rarely appeared. The Thousand Hands Clan's Pillars worked hard to persuade the Uchiha Clan's Ban to reach an agreement to stop the war, and they were hired by the Fire Country to build the Koba Hidden Village.
The Thousand Hand Clan had Thousand Hand Buddha. He was the Clan Leader of the Thousand Hand Clan and the father of the four brothers, Thousand Hand Pillar, Thousand Hand Door, Thousand Hand Tile, and Thousand Hand Hand Tile. The first generation of Huoying was Thousand Hand Pillar. The second generation of Huoying was Thousand Hand Door. Thousand Hand Tile was the younger brother of Pillar and Door, the older brother of Thousand Hand Tile, and the third son of Thousand Hand Buddha. Thousand Hand Tile was the granddaughter of Pillar. She was one of the legendary Three Ninjas and was also the fifth generation of Huoying.
The known members of the Thousand Hand Clan were Thousand Hand Buddha, Thousand Hand Pillar, Thousand Hand Door, Thousand Hand Tile, Thousand Hand Hand Palm, and Tsunashou.