Perhaps there is an ancient evil that has been awakened by the presence of Harry and the vixen's magic. This entity, which was once sealed away, now sees Harry as a way to regain its full power and spread chaos in the wizarding world. It would target Harry specifically because of his special relationship with the vixen and the unique magic he has inherited.
It could be another magical creature or group that is jealous of the power that the vixen holds and by extension, Harry as her adopted son. Maybe there is a group of dark wizards who want to harness the vixen's magic through Harry, and they become the antagonists as they try to capture him.
One possible main antagonist could be Voldemort still. Even though Harry is now under Sparda's protection, Voldemort would see Harry as a threat and would try to get rid of him. Another could be some of the demons from Sparda's world who see Harry as an intruder or a threat to their plans in the new world order that Sparda is trying to create with Harry by his side.
Definitely Voldemort. He's the main baddie in the Harry Potter series. But in this fanfiction, there could be other villains too. Maybe some jealous Disney characters who don't like Harry getting all of Mickey's attention. Or some dark forces from the edges of both the wizarding and Disney worlds that team up with Voldemort to stop Harry and Mickey's new family dynamic.
It could be the other Titans who don't want a half - wizard, half - Titan like Harry to exist. They might see him as a threat to their pure Titan bloodlines or their power structure.
The Death Eaters would be obvious antagonists as they are in the original Harry Potter series. But also, there could be a group of shadowy figures from Nightwing's world who oppose the idea of a wizard - Nightwing hybrid. They see Harry as an abomination and try to eliminate him before he can fully realize his powers.
There could also be some characters from the Doctor's universe who oppose the idea of a half - wizard, half - Time Lord. They might come to the wizarding world to try and stop Harry from using his combined powers, fearing it could disrupt the balance between the two universes.
It could be some dark wizards who are threatened by Harry's unique heritage. They might see him as a potential threat to their power and plans. Maybe they want to use his connection to Spike for their own evil purposes and when Harry refuses, they become his enemies.
Voldemort or his followers could still be antagonists. They might not like the idea of Harry being under Euryale's protection as it could make him more powerful. Another possibility could be some creatures from Euryale's world who are against her taking in an outsider like Harry.
The Joker could be a main antagonist. He would be fascinated by Harry's magic and would try to use it for his own evil purposes or just to cause chaos. He might try to drive a wedge between Harry and Batman by using mind games.
It could be some jealous rivals of Arcuid. They might see Harry as an outsider and a threat, especially if they think Arcuid is giving Harry special treatment or powers. Maybe there are those who want to use Harry against Arcuid for their own gain.
The main antagonists could still be the Death Eaters. Since they are the traditional enemies in the Harry Potter universe. They would see Harry, even as the son of Ziva David, as a threat to their dark plans. They might target him not only because of his connection to Harry Potter but also because they fear the unknown elements that Ziva's heritage could bring into the fight against them.