Maybe another kitsune who has gone bad. In the fanfiction world, there could be a rogue kitsune who sees Naruto as a threat. This antagonist kitsune might be jealous of Naruto's power or his connection to the human world. They could try to undermine Naruto's attempts to protect the village or his friends.
A dark wizard or sorcerer could also be the main antagonist. They might be after the unique power that Naruto, as a kitsune - ninja hybrid, possesses. This antagonist could use dark magic to try and capture Naruto and use his power for their own evil purposes, like taking over the ninja world or awakening some ancient evil.
It could be an evil shinobi who wants to capture the Kitsune Anbu for their unique powers. Maybe they want to use those powers to take over the Hidden Leaf Village. Another option is a rogue Anbu member who has turned against the village and sees the Kitsune Anbu as a threat to their own plans.
It could be another powerful kitsune who wants to use the assassin for their own evil plans. This other kitsune might be jealous of the assassin's skills and wants to control them.
Obviously, Naruto would be one of the main characters. He's the protagonist of the Naruto series and would be the main link to the ninja world in this crossover. Another could be a young Kitsune, maybe one that's just coming of age in the Kitsune world. Their paths cross, and they have to learn to work together.
Perhaps an ancient evil that has been sealed away in the empire for centuries but is released due to the chaos of the crossover. This entity could be more powerful than any foe the Naruto characters have faced before, forcing them to find new ways to fight.
A group of rogue shinobi and some of Daria's school bullies could team up. The shinobi would give the bullies some ninja powers, and together they would cause trouble in both worlds. This group would be difficult to deal with as they combine the physical strength of the shinobi with the local knowledge of the bullies.
Another possibility is a corrupted dragon spirit that has been awakened due to the mixing of the two worlds. This spirit could be spreading chaos and trying to destroy both the ninja world and the dragon world. Naruto would have to figure out a way to stop it, as it has powers that are beyond what he has faced before.
It could be an evil ninja from Naruto's world who somehow gains Kryptonian - like powers. He becomes power - hungry and decides to take over both the Naruto world and Supergirl's world. His knowledge of ninja techniques combined with super strength and other abilities would make him a formidable foe.
Madara Uchiha would also make a great antagonist. Imagine if he was resurrected and saw Naruto with Samehada as a threat to his plans of creating a new world order. He would use his Sharingan and Rinnegan powers to try and take Samehada from Naruto, believing that with it, he could easily defeat all who oppose him and reshape the ninja world according to his will.
It could be a powerful dark spirit from Kagome's world that somehow manages to cross over to the Naruto universe and starts causing chaos. This spirit might be immune to normal ninja attacks and require Kagome's spiritual powers to be defeated.
Perhaps an ancient and evil ninja from a long - lost clan that was sealed away behind the gate. He resents the modern ninja world and wants to destroy it. He has unique and powerful jutsus that Naruto and his friends have never seen before.