The main antagonist could also be a new character created specifically for the fanfic. This character could be an ancient evil that has been waiting for an opportunity to corrupt a powerful Huntress like Ruby. They would then use Ruby's newfound evil nature to further their own dark plans, and Weiss, Blake, and Yang would have to face both Ruby and this new threat.
Another possibility is that the antagonist could be Ruby herself in a sense. Her inner demons could be personified. As she turns evil, her own good side could be fighting against the darkness within her. So, her former self or the part of her that still has some goodness left could be the 'antagonist' as it tries to prevent the complete takeover of the evil Ruby.
If Ruby turns evil, she could become her own antagonist in a way. Her former self, the Ruby that was good, could be seen as what she is now fighting against. Her friends would also become antagonists as they try to stop her from causing more harm.
Perhaps it could be an evil version of one of Ruby's own relatives. Like an alternate version of her father or mother who has been corrupted by some dark force. They would then raise Ruby with their evil values, making her believe that what they do is right and leading her down a dark path.
One possible plot could be Ruby being betrayed by her closest friends. Maybe she discovers a dark secret about the Huntsman academies that makes her question everything she has ever believed in. This disillusionment could start her on the path to evil as she feels that the system she has dedicated her life to is corrupt.
Well, one possibility is that Ruby could be influenced by a powerful and evil relic. Maybe she discovers a dark side of the relic's power and becomes corrupted by it, gradually turning evil as she starts to value power over her friends and the greater good.
It could be a powerful Grimm - like being that has evolved beyond the normal Grimm. This antagonist might have the ability to think and strategize like a human, making it a much more dangerous foe for Ruby and her team. It could be leading the Grimm attacks in a more organized way, targeting specific areas to cause maximum chaos.
It could be Yuno. Normally they are friends, but if Asta turns evil, Yuno might be the one to oppose him. Yuno has his own strong magic and a sense of justice that would make him a natural adversary.
It could be Salem. She has always been interested in the power of the silver eyes, and Ruby having those eyes makes her a prime target. Salem might send her minions to kidnap Ruby, which would lead to her disappearance.
The Spirit of the Millennium Items could also be the main antagonist. They were initially in harmony with Atem, but with his turn to evil, they might try to resist his control. Since they are powerful entities in their own right, they could become a force against Atem. They would not want to be used for evil purposes and would fight back against Atem's newfound malevolence.
It could be Adam Taurus. He's a well - known and very radical member of the White Fang in the RWBY universe. His extreme views and violent methods would put him directly at odds with Ruby's more peaceful and inclusive approach.
Another possibility is that Twilight herself could be the main antagonist in her own internal struggle. As she turns evil, there could be a part of her that still resists this change deep down. So, the evil side of Twilight that is taking over could be seen as the antagonist to the good side of her that is fighting to regain control.