It could be Orochimaru. He has always been a major threat in the Naruto universe. If Sakura is on a mission while wearing the mask - like Kakashi, Orochimaru might be interested in her new look or the power she seems to be emulating. He could try to capture her to study her abilities or use her for his own evil purposes.
The Akatsuki members could also be the main antagonists. They are always on the lookout for strong ninja. If Sakura is wearing the mask and acting more like Kakashi, they might mistake her for a more powerful threat than she actually is (at first). So, they could target her, leading to a series of battles and challenges for Sakura in the fanfiction.
Perhaps in this fanfiction, Sakura wearing the mask - like Kakashi is a symbol of her growth. She might be emulating Kakashi's calm and collected demeanor. It could start with her being inspired by Kakashi's success in various missions. So, she decides to wear a similar mask to see if she can also gain an edge in her own missions, both in terms of physical combat and mental strategy.
It could be Orochimaru. He might be interested in the baby for some dark experiment, thinking the baby has unique chakra or abilities that he can use for his own gain. He would stop at nothing to get his hands on the baby, which would put Kakashi and Sakura in a tough situation to protect it.
Itachi himself could be seen as the main antagonist in this scenario as he is the one carrying out the kidnapping. He is acting against Sakura's will and causing a dangerous situation for her.
Kakuzu might also be a main antagonist in this fanfiction. He's always after money and power. Maybe he sees Sakura as a valuable asset, either to sell her back to Konoha for a large sum or to use her skills for his own gain. He's not very empathetic and would likely be ruthless in his treatment of her.
It could be Orochimaru. He's always had an interest in powerful shinobi, and Sakura has shown great potential. Itachi might be roping Sakura's wrists to prevent Orochimaru from getting to her and using her for his evil experiments.
In this case, Sakura could be seen as the antagonist, at least in that particular moment when Naruto tells her off. Since Naruto is the one doing the telling - off, it's because of something Sakura has done or said that has pushed him to that point.
Perhaps an old enemy of Sasuke's family, someone who holds a grudge against the Uchiha clan. They might see the new baby as a continuation of the Uchiha line and try to end it. This would put Sakura and Sasuke in a difficult situation where they have to use all their skills and the help of their friends to protect their baby.
Itachi himself could be seen as the main antagonist in this scenario as he is the one performing the kidnapping. His actions are against Sakura's will and are likely to cause distress and conflict.
Perhaps there is no traditional antagonist. Instead, Naruto's own insecurities could be the 'antagonist'. He's so used to wearing the mask of the happy - go - lucky guy that his inner self is resistant to being exposed. So, in a way, his own fear of change and vulnerability is what Sakura has to fight against when she tries to break his mask.
Maybe an organization that wants to use her unique abilities. If she has inherited some special power from Kakashi or has her own distinct skills, an evil organization could try to capture her and force her to work for them. They would be the main obstacle she has to face in the story.