It could be someone who wants to expose Naruto's secret. Maybe a rogue ninja who wants to use this information to create chaos in the village by making the villagers doubt Naruto's intentions.
Perhaps an old enemy of the Hokage lineage. They might sense that something is different about Naruto and suspect he has a connection to the Hokage. This antagonist could try to stop Naruto from achieving his goals related to being a great Hokage, like preventing him from learning important jutsu or making alliances.
There could be a power - hungry council member in the village who doesn't want Naruto to be the Hokage, even secretly. They might try to undermine his secret leadership by spreading false rumors or causing political unrest within the village.
It could be a rogue ninja who was once a part of the Akatsuki but managed to escape before their downfall. He's been biding his time, growing stronger, and now he wants to take revenge on Naruto and the Hidden Leaf for what happened to the Akatsuki.
A group of rogue ninjas from other clans who oppose the idea of the Hidden Leaf Village. They see the Shodai Hokage's leadership as a threat to their own power and freedom. They might team up and use underhanded tactics like assassinations, sabotage of the village's resources, and spreading false rumors to try and undermine the Shodai Hokage's authority. They could also form alliances with other enemies of the village, making them a formidable and complex antagonist group.
It could be a rogue ninja who was once a part of Konoha but was banished for unethical experiments. Now, with a thirst for revenge against Naruto and the village, he returns as a powerful and unpredictable foe.
Perhaps an old enemy of the Sannin resurfaces. This antagonist could see Naruto's attempts to follow in the Sannin's footsteps as a threat. They might target Naruto to get revenge on the Sannin or to prevent a new powerful force from emerging in the ninja world. For example, if there were a group that was once defeated by the combined efforts of the Sannin, they could try to stop Naruto before he becomes as strong as the Sannin were together.
It could be a rogue shinobi from Naruto's past who has been biding his time. This shinobi might have developed a new jutsu specifically designed to target Naruto and make him die slowly. He's doing it secretly to cause chaos in the Hidden Leaf when their hero suddenly passes away.
It could be a rogue ninja from another village who has a vendetta against Konoha. Maybe he blames the Godaime Hokage for something that happened in the past, like the death of his family during a Konoha - related mission. So, he decides to target the village and the Godaime Hokage in particular.
Perhaps the main antagonist is a spirit or a curse that has been awakened. This curse could be slowly influencing the villagers to turn against Naruto. It could be something that was sealed away long ago and now has broken free due to some mistake or interference. The antagonist could also be a mastermind from another hidden village who is using spies and psychological warfare to undermine Naruto's rule as the Sixth Hokage.
Perhaps an old organization that was thought to be defeated resurfaces. They were biding their time while Naruto was hokage, and now that he's retired, they believe they can carry out their evil plans. They could be a group with a long - standing vendetta against Konoha and Naruto specifically. They might have new members or new powers that Naruto and his friends have to figure out how to counter.
Maybe Naruto has always idolized the Hokage in secret. He could be studying the ways of past Hokages on his own, trying to learn their leadership skills and jutsu. For example, he might be secretly practicing Minato's Flying Thunder God Technique because he admires his father so much.
Perhaps a jealous rival. This person might be a male who is threatened by Ed's success or popularity. When they discover Ed is a girl, they could use this secret to try and drive her out of the group or community she's in. They might spread rumors or create problems for her.