In some cases, it could be a combination of characters. For example, a group of older and more traditional Fairy Tail members who don't like the new and different ways of the OC. They work together to isolate the OC, making them feel betrayed. So, this group of conservative guild members could be seen as the antagonists.
It could be Zeref. Since he has a complex relationship with Natsu in the original story, he might manipulate things behind the scenes to make the Fairy Tail guild betray Natsu. Maybe he wants to isolate Natsu for his own evil plans.
It could be an ancient evil entity that has been sealed away for centuries. The OC's rebirth somehow disturbs the seal, and this entity awakens. It views the Fairy Tail guild as a threat because of their positive influence in the world. So, it starts to target the guild, and especially the OC, as they are a new and unknown factor in the fight against it.
It could be a jealous Fairy Tail member. Maybe someone who is envious of Naruto's unique powers and the attention he gets from some of the guild members. This person could start spreading lies about Naruto to turn the others against him.
Well, perhaps the OC was wrongly accused of stealing a powerful magic item from the guild. The other members turned against the OC without really listening to their side of the story. Then the OC, feeling betrayed, decides to leave Fairy Tail and seek revenge or prove their innocence on their own.
A powerful dark mage who was once a member of Fairy Tail but was banished for using forbidden magic. This mage now wants revenge on the guild and sees the reborn OC as a threat to his plans. He has the ability to control shadows and can create illusions to deceive the members of Fairy Tail.
It could be a former Fairy Tail member who turned to the gangster life. They know all the guild's secrets and weaknesses, so they can be a very formidable opponent. They might have left the guild because they felt betrayed or didn't get the recognition they thought they deserved.
It could be an ancient dragon that has been awakened from a long slumber. This dragon might have a vendetta against Ignia or the Fairy Tail guild for some past transgression.
A group of rogue mages who oppose the idea of Yukino joining Fairy Tail. They might view Fairy Tail as a threat to their own plans in the magical world. They target Yukino as the 'newcomer' in the guild, thinking that if they can get rid of her, they can weaken Fairy Tail. They use various underhanded tactics like spreading false rumors about Yukino within the guild and attacking her when she is on solo missions.
It could be Natsu. He's hot - headed and might not understand Naruto's ways at first. He could see Naruto as a threat to his position in the Fairy Tail guild and start bullying him to assert his dominance.
Zeref could be a great main antagonist. His long - standing connection to dark magic and his complex relationship with the Fairy Tail characters make him a powerful and menacing figure. He could be the one orchestrating the universal war in an attempt to finally achieve his dark goals.