Maybe a mysterious organization that wants to experiment on Latios. They believe that Latios holds the key to some sort of genetic or power - enhancing technology. They send out their agents, who are skilled in Pokémon capture and combat, to try and get Latios. They don't care about Ash at first, but when he stands in their way, they see him as an obstacle to be removed.
Perhaps a dark - type Pokémon that is jealous of Ash's new form. This Pokémon might see Ash as a threat and constantly try to attack him and stop him from getting used to his Latios form.
One possible plot could be that Ash meets a Latios in a mysterious forest. Latios is injured, and Ash decides to help it. As he nurses it back to health, they form a strong bond. Ash then discovers that Latios has a special mission related to protecting a hidden power in the forest. With the help of his Pokémon, Ash joins Latios on this mission, facing various challenges along the way.
A plotline might be that Latios is from a different dimension or time, and it has come to Ash's world for a specific purpose. Ash gets involved in this mission, and together they have to face powerful enemies. This could introduce new Pokémon species from other dimensions and new battle strategies that Ash has to learn to keep up with the challenges.
Latios would obviously be a key character. Also, Pikachu, Ash's loyal Pokémon partner, would be there. Maybe Misty or Brock could also be part of the story, with them either supporting or being skeptical of Ash's newfound admiration for Latios.
Well, I haven't really read a particular 'ash starts with latios fanfiction' myself. However, if you join some Pokémon - related fan groups on social media platforms, like Pokémon fan clubs on Facebook or Reddit communities dedicated to Pokémon fanfiction. You could ask the members there if they know any good ones. They are often very helpful in sharing their favorite fanfictions.
It could be a jealous rival trainer who mocks Ash for wearing a dress and tries to use it as a way to undermine his confidence and stop him from competing in Pokemon battles.
May could be one of the main characters. She could join Ash on his journey and share her knowledge about Hoenn's Pokemon with him. Another could be Brock, still tagging along to offer his expertise in Pokemon care.
A powerful and evil Team Rocket member could be the main antagonist. This person might be after Lucario because of its rare abilities. They could try to capture Lucario to use it for their own evil plans, like taking over the Pokémon world or stealing valuable Pokémon. Ash would then have to fight against this Team Rocket member to protect Lucario.
A jealous rival who also wants to be known as the dragon master. This person would try to undermine Ash at every turn, spreading false rumors about him and trying to steal his dragon - taming techniques.
Maybe Team Rocket could be the main antagonist. They might see Ash's transformation as an opportunity to finally capture Pikachu. Since Ash is in a more vulnerable state as a girl, they could try to use different tactics to take advantage of the situation.