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Mo Fan and Song Qiming were members of the Clearsky Hunter Agency. Song Qiming was the founder of the Clearsky Hunter Agency, and Mo Fan was hired by him. While waiting for the TV series, you can also click on the link below to read the classic original work of "Dafeng Nightwatchman"!

The following are some of the answers to the 2020 National Middle School Biology League questions: 1. In the traditional Trizol method for extracting the DNA, the following compounds were used: - The main components of Trizol were carbolic acid and guanidinium thionate. The carbolic acid could dissolve the protein and lyse the cells, releasing the protein/nuclear acid substances. Therefore, option A was correct. - The function of the chopstick was to help the separation of the water phase from the organic phase and to remove the compounds in the solution, so option B was correct. - Chloric acid didn't denature the DNA. Option C was wrong. - Chloric acid could remove some fat-dissolved impurities in the sample, such as oil and fat-dissolved colors. Option D was correct. Therefore, the answer to this question was an ABD. 2. Regarding the secondary structure of the green fluorescent protein, the green fluorescent protein was surrounded by a cylindrical fence: - The region where the larger R's (such as phe, pt, and ip) were concentrated would hinder the formation of the alpha helices, so option A was wrong. - The side chain of gly was too small, so its conformations were unstable and destroyed the formation of the alpha helices. Option B was wrong. - Phe can form a beta-sheet. Option C is correct. - The peptidic chain contains a protein that can form a beta-sheet. Option D is correct. Therefore, the answer to this question was CD. 3. The purification method of the Green Phosphorescence from the E. Coli lysed solution containing the Green Phosphorescence protein with the N-terminal 6*His tag: - According to the difference in the surface of the protein, the interaction between the protein and the surface of the medium was used to separate the protein. - Ion exchange separation of protein was based on the different charges of the protein under a certain pH. - Immobilized metal ion affinity column was a method for separating and purifying His-tag protein. This gel could specifically chelate with the His-tag protein, so that the protein was firmly attached to the gel through the metal ion. Other impurity protein could not be chelated with the metal ion because it did not carry the purification tag, so it could not be attached to the gel. - Gel-phase separation was a technique that used the molecular sieve effect of the particles with a multi-pore network structure to perform elution-separation based on the difference in the relative molecular mass of the components in the sample to be separated. Therefore, immobilized metal ion affinity column was the first choice for purification of the GFlp from the E. Coli lysed solution containing the GFlp protein with the N-terminal 6*His tag. The answer was C. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

Here are some reference novels where women fall into the boss's trap: - In a workplace novel, a female boss was a tyrant who always made things difficult for her subordinates. The subordinate accidentally heard the bribery transaction of the female boss and collected evidence to report it. In the end, he was framed. Later, he found out that the account book of the female boss's house was turned over. This was the story of the female subordinate falling into the trap of the female boss. - There was also a story about a woman and her former boss. The woman asked her former boss about how to make money. The boss was deeply mired in online gambling and fabricated lies about e-commerce to cheat the woman into investing. The woman invested more than 250,000 yuan and found out that she was cheated. This was the story of the woman falling into the trap of the former boss. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

From the reference materials, he could obtain some sentences that reflected the scenes in the novel into reality, such as " It turns out that everyone likes to imitate novels. The entertainment industry exploded. I only felt relieved when I saw this video. So I'm not the only one who reads novels like this. I always thought that imitating the expressions of the characters in novels was a very niche hobby of mine. I didn't expect everyone to be like this." This reflected the fact that people imitated the actions of the characters in novels in reality. Also," The CEO in the novel is either busy courting his wife or abusing his wife every day. In reality, the CEO is so busy that he can't be seen." It reflected the difference and connection between the image of the president in the novel and reality. It was also a manifestation of the novel's image shining into reality. From the comparison, it reflected people's thoughts on the relationship between the novel and reality. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

The size of the B5 paper depends on the precision of the output. When the standard printing was 300dpi, there was no explicit mention of the exact data of the B5 paper's size. However, you can refer to the data of the relevant paper: A4 is 2480* 3508, A3 is 4960*3508, B3 is 3248*4300, and B4 is 3248*2150. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

Aerobic breathing referred to the process in which cells, with the participation of oxygen, completely oxided and decomposed organic substances such as glucose into carbon dioxide and water, releasing energy and generating a large amount of Ang. Aerobic breathing was the main form of breathing for higher animals and plants. Usually, the so-called breathing referred to the use of oxygen. Aerobic breathing was carried out in the cellular matrix and the mitochondria, and the mitochondria was the main place for cells to carry out oxygen breathing. It could be divided into three stages. The first stage was the initial decomposition of glucose in the cellular matrix. One molecular sugar was decomposed into two molecules of methyruvate, four activated hydrogen (H), and a small amount of ATP. The second stage was the complete decomposition of the Pyruvate in the mitochondria matrix. The hydrogen in the two molecules of Pyruvate and the six water molecules was completely removed. The Pyruvate was then oxided and decomposed into carbon dioxide, producing a small amount of energy. The third stage was the combination of the hydrogen and oxygen removed in the previous two stages into water on the membrane of the mitochondria, releasing a large amount of energy. The fate of the energy includes storage in the form of active chemical energy in the ATPs and dissipation in the form of heat. The complete decomposition of 1 mole of glucose in the body can release 2870kJ of energy, of which about 1161kJ of energy is stored in the ATP-bound substance (about 38 moles of ATP-bound substance). The energy utilization rate is about 40.45%, and the rest of the energy is lost in the form of heat energy. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

In terms of social class, primary school teachers were often children of ordinary families. In the past, due to the relatively low entry requirements for teachers and the fact that they could be assigned jobs after graduating from technical secondary school, students with poor family backgrounds chose to be teachers. At present, although the threshold for teachers had been raised, on the whole, the income level of primary school teachers was within a certain range (for example, the income of ordinary teachers in public primary and secondary schools was about 180,000 to 300,000 yuan, and the income of ordinary private aristocratic school teachers was about 50,000 to 120,000 yuan). Their social status was not very high, and there was greater competition, and their happiness was low. And those families with better family backgrounds, connections, and money usually wouldn't let their children choose the profession of primary school teachers. From the perspective of the internal class of the profession, primary school teachers were in a lower position in the contempt chain of the teaching industry (university teachers> high school> junior high school> primary school> kindergarten), facing internal pressure in terms of education, age, professional title, family background, etc. For example, in terms of academic qualifications, there were fewer primary school teachers in some small places with postgraduate qualifications, because higher academic qualifications would have more career choices; In terms of age, young teachers and older teachers had different ideas and were criticized; In terms of professional title evaluation, it was more difficult for ordinary first-line teachers with teaching ability to be awarded senior professional titles; In terms of family background, primary school teachers were mostly female and their income was relatively low, so there was a phenomenon of comparison. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

Huaxi Biology's stock price reached a historical high of 313.1 yuan (2021). <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

The following are some single lithium battery power detection chips: - For example, Rhyme's CC1185TSSOP 16 single-cell lithium battery integrated circuit power display detection chip. In terms of price, the starting batch of 10 PCs was $1.50, 1000 - 3999 PCs was $1.48, and 4000 PCs was $1.46. The shipping place was in Guangdong Province, and the courier fee was $24.00. - The FH5060 was a single-cell lithium battery power indicator chip, implemented in the complementary metal fabricating process. It was small in size and easy to install in portable products. It has a built-in comparer and feedback loop, which can detect four voltage points. Through internal trimming technology, the voltage detection accuracy can reach +/-1%. The output uses the open-drain structure, which is easy to use the I/O port or LED indication. It has ultra-low power consumption (< 10.0uA) and other characteristics. It is suitable for mobile power supplies, LED flashlights, portable electronic devices and other fields. - YJ60A was a single-cell lithium battery power indicator chip that could detect four voltage points. - The single-cell lithium battery charging protection chip MX2201B has a built-in high-precision voltage detection circuit and delay circuit, as well as a built-in upheaval. It is used for the protection of single-cell lithium-ion/lithium-poly-charged batteries. - The PW5306 is a multi-functional power management SOC. It has an integrated output 5V boost conversion, an input 5V buck lithium battery charging manager, and a battery level indicator controller. It provides a convenient and simple solution for lithium battery applications. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>

The prospects of the biological science major were multi-dimensional. On the positive side, biological science was closely related to human life. Many of the major problems faced by mankind depended on the progress and development of biological science and biotechnology to a large extent. For example, in the field of medicine, with the development of biological science, the early detection, early intervention, and early treatment of tumors were increasing. The concept of survival with tumors was gradually accepted by more people, and diseases such as Alzheimer's disease could also be delayed. In agriculture, the future agriculture and marine agriculture that were friendly to the environment and resource-saving had potential for development. The protein structure and source of food might be more inclined to be obtained from the ocean. The biological science major provided students with a broad knowledge background, covering a variety of subject knowledge, thus providing students with rich employment opportunities. Moreover, the biological science major students had good scientific literacy after relevant training, and had preliminary teaching, research, development, and management abilities. However, biological science majors also faced some challenges. In terms of employment, there were some difficulties in choosing a profession for this major. If one were to be a technician, their income would be limited and they would be mainly responsible for production in the laboratory. The chances of research and development would be slim. Even doctors from famous universities would mostly be engaged in production and technical supervision. If he went to a research institute to apply for a technician or experimental assistant, the job would be monotonous and the requirements for the technical mastery of the undergraduate stage would be high. His future would not be bright. There were fewer positions in the biological science major, and the competition was fierce. In the employment of enterprises, the better way out for biological science students in ordinary colleges was to work in public institutions and state-owned enterprises. In general, students who majored in biological sciences could make use of the school's resource platform to further their studies and accumulate work experience to find a suitable direction for themselves if they wanted to develop in their field. If they wanted to engage in other industries, the various abilities and extensive knowledge background cultivated by the biological sciences major could also be used as a career change. <a href="/?from=ask_words" style="color:red" target="_blank">Read more exciting novels for free</a>