cultural relic extractionThe extraction of cultural relics was divided into different situations. The following were some common methods of extracting cultural relics:
** 1. Underwater archaeological fragile cultural relics extraction **
1. ** Supporting or holding conventional utensils **
- For fragile cultural relics in underwater archaeology, different instruments were usually used to support or contain them, such as covered containers with protective foam lining, polythene boxes, ziplock bags, etc. For fragile cultural relics such as large underwater condensation, they were mainly extracted by metal frames.
2. ** Reinforcement Technique **
- In the implementation of the SASMap project, the National Museum of Denmark designed and used bandage wrapping, carbon fiber/epoxide-based wrapping, and liquid nitrogen freezing to reinforce underwater fragile cultural relics.
- The State Administration of Cultural Heritage's archaeological research center, together with Zhejiang University and Beijing University of Science and Technology, jointly developed new technologies such as the veratrolum reinforcement material and the underwater epoxide-based multi-layer material solidifying system. Veratrin had a low melting point (40 - 43 ° C) and a density greater than seawater (1.1708 g/cm - 3). It was almost impossible to dissolve in cold water, but it was easily dissolved in common organic reagents such as alcohol at room temperature. It could be used to solidify underwater to strengthen fragile cultural relics, and it was easy to remove without leaving any residue. After the cultural relic is extracted, the mixed solution of alcohol and water can be used to soak and remove the veratrolic acid, and the removal rate can be adjusted by adjusting the addition ratio of alcohol. The method has little influence on the cultural relic, and can ensure the safety of subsequent desalination, dehydration, removal of coagulate and restoration work of the cultural relic.
** 2. Extracting archaeological relics on land **
1. ** Basic extraction method (suitable for situations where the soil condition is good and the cultural relics are small)**
- Removing the surrounding soil: It was not to completely remove the soil around the cultural relics, but to remove the soil surrounding the cultural relics so that the cultural relics could be placed on a safe soil platform. The soil platform for carrying cultural relics is usually rectangular, and when the soil strength reaches the standard, it can be turned into an inverted echelon.
- [Surrounding reinforcement mode: Use reinforcement materials to carry out simple reinforcement operations around the cultural relic soil platform. It is mainly temporary reinforcement. The basic operation modes include gauze bandage method, plaster bandage method, and resin bandage method.]
- Undercutting: Use a metal plate or metal wire with a blade at one end to divide the platform base along the horizontal position at the bottom of the platform base that carries the cultural relics. Then, separate the platform base from the ground on this basis. During the process, it cannot shake or pry.
- [Rigidity Support: Choose a metal plate with a blade at one end to perform undercutting. After the undercutting is completed, the metal plate can be used as a rigid support structure.] In the whole extraction process, after the undercutting treatment, the whole extraction block should be slid to the rigid support plate. The rigid support plate should be used to process the horizontal position of the undercutting treatment plate to extract the cultural relic, and the other end should be used to place the cultural relic on the treatment plate.
2. ** Box Extraction Method **
- [Remove the surrounding soil: Use only rectangular foundations.]
- Surrounding reinforcement: The main use is to tightly wrap the soil with a wooden frame. If there is no suitable wooden frame material, you can use a flat surface template to surround the soil, and then use bandages to tie it in a suitable position on the outside. You must also maintain the tightness between the inside and the side of the platform template.
In different archaeological scenarios, the appropriate extraction method would be selected according to the characteristics of the cultural relics, the environment, and other factors. The purpose was to protect the cultural relics to the greatest extent, maintain their original state, and not destroy the cultural relics and the information carried by the cultural relics themselves.
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Music accompaniment extractionHere are some methods to extract music accompaniment:
** I. Software and methods suitable for professional users **
1. **Adobe Audition**
- It was a professional audio editing and mixing software developed by the company, specifically designed for audio and video professionals.
- You can extract the accompaniment by installing the Musical Remover plug-in. The specific operation was to first open the software, import the song audio file that needed to extract the accompaniment, then apply the Vocal Remover plug-in on the audio track, adjust the parameters to achieve the best accompaniment effect, and finally export the processed audio file into the desired format.
2. **Audacity**
- This was an open source audio editing and recording software that provided advanced functions such as spectrum analysis and vocal track separation, which could be used for accompaniment extraction. It had a series of audio editing tools such as cut, copy, paste, drag adjustment, etc., which could accurately edit the audio. It also supported the installation and use of third-party plug-ins, and users could expand the software functions according to their needs.
3. ** FFmux (suitable for professional users on Windows)**
- On Windows, you need to open the command prompt (Command Prompt) or PowerShell to operate, but the specific steps do not explicitly mention how to extract the accompaniment, only that it can be used for this purpose.
** 2. Software and methods suitable for Xiaobai users (including mobile phone users)**
1. ** Easy for me to separate from the human voice **
- It was an AI tool developed by a well-known domestic software manufacturer, Yiwo Technology, that could be used online.
- Operation method:
- Web side: Enter the official website, click the "extract now" button and jump to the function page. You can choose the audio file to be separated. In addition to voice separation, you can also separate the instrument and eliminate noise. Common video and audio format are supported. After selecting the file and waiting for processing, you can get the separation result and then save it to the computer.
- Mobile: You can search for the Mini programs "Easy Voice Separation" in the green bubble, click on it to select the local file to import the audio file that needs to be separated (usually the first voice and accompaniment option is used), click on the bottom to start separation, and after the separation is completed, directly export the results. If you want to save it, you need to copy the link to the built-in browser and save it to the phone album. Then you can import it in the editing software.
2. **LALAL.AI**
- It was a foreign open source online audio separation website that used AI artificial intelligence audio separation technology to support the separation of vocal, instrument, bass, electric guitar, and other musical instrument tracks.
3. ** Jinzhou series of apps (suitable for mobile users)**
- ** Jinzhou audio and voice separation APP**: 9.8 points. Its functions include audio accompaniment extraction, voice extraction, video accompaniment extraction, voice extraction, audio format conversion, video to audio conversion, etc. The operation steps were to enter the software, select [audio extraction accompaniment], select the audio, set the export format [mp3], and complete the processing.
- ** Jinzhou format factory APP**: 9.8 points. It can solve problems such as audio accompaniment extraction, human voice extraction, video accompaniment extraction, human voice extraction, audio format conversion, audio merging, cutting, etc. The operation steps were to enter the software, select [audio extraction voice], select the audio, set the export format [mp3], and complete the processing.
- ** Jinzhou MP3 Convertor APP**: 9.2 points. It focuses on audio extraction and can easily separate audio and video. It also has functions such as audio format conversion and audio editing. The operation steps were to select [Video to audio] on the Jinzhou MP3 audio changer page, select the video to extract the audio, and extract it to complete the audition.
- ** Jinzhou audio editing and conversion APP**: Rating 9.2. It is a professional audio editing software. Not only does it have the function of extracting video accompaniment, but it can also cut and convert audio. It is easy to operate and suitable for music production enthusiasts. The operation steps were to select [Video to audio], select the video, select the export format, mp3, and save it.
- ** Music and audio editing APP**: 8.9 points. Not only can it extract audio from various video format, but it also supports audio format conversion, volume and speed adjustment, audio merging, audio trimming, and other functions. Its audio editing function supports professional editing in the millisecond-level. The operation steps were to select [Accompanying Extraction], select the music, select the export format, mp3, and save it.
- ** Audition Master (similar to Audition Master)**: Rating 8.5. It is a full-featured audio processing software, including the voice separation function. The operation steps were to select [Accompanying/Voice Separation], check the music, and start processing.
- ** audio extraction APP**: Rating 8.0. It's a software that focuses on audio separation and supports multiple audio files. The operation steps were to select [audio extraction], check the video, extract the audio mp3, and save it.
- ** Master of audio extraction APP**: 7.9 points, comprehensive functions, including voice separation function. The operation steps were to select [Accompanied Music Extraction], select the music, and save it.
- **MP3 audio extraction APP**: 7.5 points. It is specially designed for audio processing and supports many advanced functions, including voice separation. The operation step was to select [audio extraction].
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The Strongest Extraction SystemThe " strongest extraction system " setting was indeed very common in online novels. It usually referred to the protagonist obtaining an ability to extract the essence of other people's skills, knowledge, or items. Such a system would often allow the protagonist to become stronger in a short period of time and become the best in the story. However, the specific settings and performance of each work would be different.
As a veteran user of online novels, here are some of my views on the " Strongest Extraction System " and some common plot settings:
###Positive view:
1. [This system can bring great pleasure to the readers. Watching the protagonist easily obtain powerful abilities gives them a feeling of being a cheat.]
2. ** Plot Advancement **: The existence of the system often becomes another important driving force in addition to the main mission. The protagonist will continue to take risks in order to explore more possibilities.
3. [Diverse development: The protagonist can use the extracted abilities to enter multiple fields, increasing the richness and viewability of the story.]
###Common plot routine:
1. [Unexpected gain: The protagonist activates this mysterious system by chance.]
2. ** Initial Trial **: At the beginning, there will be some simple missions to help the protagonist familiarize himself with the system functions and gradually increase his strength.
3. [Encounter a Strong Enemy: Then, more and more powerful enemies and challenges will appear, forcing the protagonist to use the system to evolve.]
4. [Moral Choice: The process of using the system may involve moral boundaries and human nature tests, such as whether one should abuse their abilities or violate the rights of others.]
5. [Final Battle: In the end, there will always be a fierce battle. The protagonist will use the power given by the system to defeat the evil forces and achieve a happy ending in a sense.]
###Reading suggestions:
- If you are interested in this type of " cheat-type " novel, the story of " The Strongest Extraction System " might be very attractive to you.
- However, at the same time, he had to pay attention to the quality of the work. Some works might be too dependent on the system settings and ignore the character creation and the depth of the story.
All in all, the " Strongest Extraction System " was a popular element of online novels. It had its own unique charm and appeal to readers, but one had to look at its pros and cons rationally!
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Biochemical plant DNA extractionPlant DNA extraction mainly involved the following processes:
1. ** Treatment of samples **
- Taking bananas as an example, the crushing method could be used. The banana was placed in a sealed bag and mashed to destroy the cell wall. You can also weigh 10g of tender plant tissue and cut it into a mortar. Add 10ml of pre-cooled grinding buffer and add about 0.1 g of SSS, and grind it into a paste on an ice bath.
2. ** Add extract **
- For the mashed banana, add 10ml of extract. The extract is a mixture of a surfacing agent and salt. The surfacing agent can clean the cell membrane, and the salt can fully dissolve the DNA into the solution.
- For the young plant tissues that were ground into paste, add an equal volume of a mixture of chloride-isopentool (a mixture of 24ml of chloride-isopentool and 1ml of isopentool), add a stopper, shake it vigorously for 30 seconds, transfer it to a centrifugal tube, and let it stand for a moment to remove the tissue protein. Then, spin it at 4000 revolutions per minute for 5 minutes and divide it into three layers. Carefully suck the upper layer of the clear liquid into a graduated test tube, and discard the cell debris, denature protein in the middle layer, and the chloride-like in the lower layer.
3. ** Follow-up treatment **
- For example, place the test tube in a water bath at 72 ° C for 3 minutes (no more than 4 minutes) to inactivate the tissue's DNA. Then quickly take out the test tube, place it in an ice-water bath to cool it to room temperature, and add a 5-mole/L solution (extract: solution = 4:1(V/V)) to make the final concentration of the solution 1-mole/L.
- Then, an equal volume of the chloride-Isopentyl alcohol mixture was added into the large test tube again, and the mixture was shaken for 1 minute. After standing still, the mixture was spun at room temperature for 5 minutes at 4000 revolutions per minute, and the supernate was taken and placed into a small beaker.
- Use a burette to suck 95% pre-cooled alcohol and slowly add it to the surface of the beaker's supernate until the volume of alcohol is twice that of the supernate. Gently stir it with a glass rod. At this time, the nuclear acid quickly wraps around the glass rod in the form of a fiber. Then, add about 0.5ml of 10ssc (weigh 87.66g of NaCl2 and 44.12g of trisodic citrates, dissolve them separately, and make them 1000ml together) to make the final concentration of 1ssc.
- The crude DNA product could be obtained by repeating the steps of alcohol and 10ssc treatment. After that, the processed RNase-like reagent solution (25-milligram/milliliter of the reagent solution was prepared with 0.14-milligram/milliliter of NaCl-like reagent solution, 1-milligram/milliliter of HCl-like reagent solution was added, and the reagent was treated in a water bath at 80 ° C for 5 minutes before use) was added, so that the final concentration of the reagent was 50 - 70? g/milliliter. The reagent was then incubated in a water bath at 37 ° C for 30 minutes to remove the RNase-like reagent.
- Add an equal volume of a mixture of chlouroform-isopentanol, shake it in a triangular flask for 1 minute, remove the residual protein and the added RNase-protein, and then incubate it at 4000pm for 5 minutes at room temperature to collect the upper layer of the water solution. After that, the purified DNA solution was obtained by the steps of alcohol and 10ssc.
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Generic chakra "extraction" Chinese fanfic