Biological patch preparationThe preparation method of the composite biological patch is as follows: a fiber layer, a stripping cloth and a diversion net are laid in a mold from bottom to top in sequence, a sealing bag and the mold form a sealing mold system, the fiber layer, the stripping cloth and the diversion net are arranged in the sealing mold system, and the sealing mold system is connected with a diversion pipe and a vacuum pipe; A vacuum pump is used to evacuate the sealed mold system and maintain a negative pressure state, the matrix layer pulp is automatically injected into the sealed mold system through a diversion pipe, the diversion pipe is closed after the matrix layer pulp fills the entire sealed mold system, and the vacuum pump is turned off; the mold system is demolded after being frozen to obtain the fiber reinforced composite matrix, and the composite biological patch is obtained after being freeze-dried.
There is also a method of preparing a biological patch that involves performing various treatments on the sheet of umbilical cord tissue, including shaking at a specific temperature.(The temperature is 20 - 30 degrees Celsius, and the time is not less than 12 hours). Use a specific hypotonic solution.(For example, the first hypotonic solution is a trimethylol amine water solution with a pH value of 7.0 - 8.5, the second hypotonic solution is a water solution of dodecane sulfuric acid, and the hypertonic solution is a mixed water solution of caprylyl ether and potassium chloride.For example, the first hypotonic solution is a trimethylol amine water solution with a pH value of 7.0 - 8.5, the second hypotonic solution is a water solution of dodecane sulfuric acid, and the hypertonic solution is a mixed water solution of caprylyl ether and potassium chloride.For example, the first hypotonic solution is a water solution of trimethylol amine with a ph value of 7.0 - 8.5, the second hypotonic solution is a water solution of dodecane sulfuric acid, and the hypertonic solution is a mixed water solution of caprylyl ether and potassium chloride...)(including an Isotonic buffer solution and double-distilled water).(at least 3 times, no less than 15 minutes each time), and then freeze drying.(including the subsequent atmospheric pressure pre-freezing, atmospheric pressure freezing of the washed sheet of umbilical cord tissue for no less than 30 minutes at an environmental temperature of no higher than-20 ° C and no lower than-60 ° C, and vacuum drying at a temperature of no lower than-70 ° C for no less than 24 hours), and finally sterilization.(For example, radiation sterilization, the freeze-dried product is sterilized at an environmental temperature of 0 - 10 ° C).
There was another type of biological patch that included a matrix with active groups, and the matrix was modified with a Phosphoryl Choline compound. The preparation method was not mentioned in detail, but the preparation of the biological patch involved the Phosphoryl Choline compound being derived from a compound with a specific structure.
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