It could be a powerful vampire clan that doesn't want Harry, a new vampire, to disrupt their ways. They might try to either kill him or force him to join their ranks against his will. They could see him as an outsider who doesn't belong in the vampire world and a potential danger to their secrecy.
It could be an old vampire who sees Elena as a threat to his power or territory. Maybe he doesn't like the idea of a newly - turned vampire causing a stir in the vampire community and decides to take her out.
Maybe Voldemort or his followers could still be antagonists. Even though Harry is now a vampire, Voldemort might want to use his new state for his own evil purposes or simply view Harry as a nuisance that must be destroyed. Since Harry has changed, he might have new weaknesses that Voldemort could exploit.
Dumbledore could be the main antagonist. Harry might see Dumbledore as having used him all along for some grand plan. If Harry turns evil, he would want to break free from Dumbledore's influence. Dumbledore, with his power and wisdom, would then try to stop Harry from causing too much harm, thus becoming the main opposing force.
It could still be Voldemort. Even if Harry is turning to the darkside, Voldemort might see him as a threat because Harry has his own unique power. Voldemort might not want to share his control over the dark forces with Harry, so he would become the main antagonist in a fight for dominance in the dark world.
Umbridge could also be a main antagonist. She is known for her strict and often cruel rules. If she discovers Harry in his female animal form, she would likely try to use it as an excuse to further oppress him. She might see it as a sign of abnormal magic and use it to justify her actions against Harry and those she doesn't like in Hogwarts.
Maybe it's a group of purist wizards who form a secret society. They are determined to 'purify' the wizarding world by getting rid of anyone or anything that is not a normal human wizard. Harry, as a monster girl, becomes their prime target. They use dark magic and underhanded tactics to try and capture or kill Harry.
Maybe a powerful and envious Mewtwo could be the antagonist. Since Ash has now become a Mew, Mewtwo might feel threatened or think that Ash doesn't deserve such power. So, Mewtwo could try to fight Ash to prove his own superiority.
It could be a new villain who is specifically targeting Gohan because of his new form. This villain might see Gohan's transformation as a sign of weakness or an opportunity to mess with the Z - fighters. Maybe they want to use Gohan's confusion and the chaos it causes among his friends and family to their advantage.
It could be someone who wants to take advantage of Minato's new female form. Maybe a villain who has a vendetta against Minato in his original form and sees this as an opportunity to further humiliate or harm him.
Another possibility is that a group of Koopa minions could be the main antagonists. They might be under the orders of Bowser, but they have their own little schemes going on. They could target Mario - turned - girl because they think she'll be an easier target in her new form, but they are in for a big surprise when she shows her strength.