Well, Mary Jane would be one of the most affected. She has a deep emotional connection with Spider - Man. Ned, his friend from school, would be lost without him. And then there's J. Jonah Jameson. Even though he's often against Spider - Man, he might feel a strange sense of loss. Also, any villains who had a sort of twisted relationship with Spider - Man might also be affected in some way, like Venom or Green Goblin.
Even one of Spider - Man's fellow superhero friends might betray him. Maybe they get jealous of Spider - Man's popularity or feel that he is getting all the credit for their team's successes. This internal betrayal would add a lot of drama and conflict to the fanfiction story.
The main characters would likely include the gender - bended Spider - Man, of course. Then there's J. Jonah Jameson. His attitude towards a female Spider - Man could be really interesting. He might be more dismissive at first, thinking she's just a girl playing superhero. And then there are the villains. They might underestimate her too, but she'll prove them wrong. Characters like Green Goblin and Doctor Octopus would still be major antagonists, but their interactions with a female Spider - Man would be fresh.
Peter Parker as Spider - Man is obviously a popular character. He's the main protagonist. Venom is often a popular character in fanfictions as well. His dark and menacing nature contrasts with Spider - Man's heroic side. Mary Jane might also be popular as she is an important part of Spider - Man's story. Another could be Harry Osborn, as his relationship with Peter is always an interesting aspect to explore in fanfictions.
Peter Parker would be extremely affected too. Iron Man was like a mentor to him. His death could make Peter question his own abilities and whether he can continue to be a hero without Iron Man's guidance. He might also feel a sense of guilt, thinking that if he had been stronger or more experienced, he could have saved Iron Man.
Scarlet Spider himself is often very well - written. Writers can explore his dual nature, being both a hero and having his own insecurities. His relationship with Spider - Man, whether it's one of rivalry or mentorship, can be very engaging.
The main characters are, of course, Spider - Man and Nova. Spider - Man is the well - known web - slinger, with his quick wit and acrobatic skills. Nova, on the other hand, has his superhuman strength and the ability to fly. They often team up in these fanfictions to fight villains and protect the city.
Peter Parker himself is of course extremely popular. He's the main character, and fans love to explore different aspects of his life, both as Spider - Man and as a regular guy. Mary Jane is also popular. Writers often use her to add a romantic and emotional element to the stories.
It could be the Green Goblin. He's always been a major foe of Spider - Man and might be the reason for Spider - Man to fake his death, like if he's planning a huge attack and Spider - Man needs to deceive him to stop it.
Obviously, Spider - Man and Venom are going to be the main characters. Spider - Man is the one being unmasked, and Venom is the one doing the unmasking.
In this fanfiction, Spider - Man is surely a main character. For the Oast side, it might be a group of characters. Maybe there's a leader - type character from the Oast who first encounters Spider - Man. And there could also be some sidekick - like characters who help or hinder Spider - Man's journey in the Oast world. They could have unique powers or personalities that contrast or complement Spider - Man's own.