One possible plot could be that Tsunade and Jiraiya are deceived by false information about Naruto. Maybe they are made to believe that Naruto has turned to a dark side. For example, an enemy might have created false evidence of Naruto collaborating with a powerful evil force. Tsunade, being the Hokage, and Jiraiya, Naruto's mentor, would then be forced to take actions against Naruto that seem like betrayal from Naruto's perspective.
In a fanfiction scenario, it could be that Tsunade and Jiraiya are under a powerful genjutsu (illusion technique). This genjutsu makes them see Naruto as a threat to the village. So, they start to plot against him. Naruto, who is clueless about this, continues with his normal life until he suddenly faces opposition from his two most trusted people. It's a heartbreaking situation for Naruto as he tries to figure out what's going on and clear his name.
In such a fanfiction scenario, it could be due to a misunderstanding. Maybe there was false information spread about Naruto that made Jiraiya and Tsunade believe he had done something unforgivable. For example, an enemy could have framed him for a major crime against the village.
One possible plot could be that Jiraiya is forced to betray Naruto due to some external threat. Maybe an enemy has captured Jiraiya's loved ones and threatened to kill them if he doesn't turn on Naruto. Jiraiya, in a difficult position, might have to pretend to be against Naruto to protect his loved ones while secretly trying to find a way to free both Naruto and his family from the enemy's clutches.
He could also try to talk to them directly, despite the betrayal. Naruto has always believed in the power of communication and the bonds between people. He'd want to understand why they thought he was worthy of betrayal and try to convince them that they were wrong.
A possible plot could be that Naruto, under the care of Tsunade and Jiraiya, discovers new aspects of his heritage. Tsunade might know some secrets about the Uzumaki clan that she shares with Naruto. Jiraiya, being his godfather - like figure, helps him explore these new revelations. Meanwhile, the Akatsuki could still be a threat, and Naruto, with his new skills and knowledge, has to fight against them to protect the village and his loved ones.
One possible plot could be that Jiraiya and Tsunade decide to adopt Naruto after seeing his loneliness and potential. They start training him not just in ninja skills but also in life values. Jiraiya might teach him more about the toad sage techniques, while Tsunade could help him with his chakra control and medical ninjutsu knowledge. Naruto would grow up in a more stable family - like environment, which would change his personality a bit, making him more confident and less brash as he has the support and love of two powerful ninjas.
He would probably also feel angry. After all, he has sacrificed so much for the village and for them. He might start to question his own beliefs and values. But Naruto being Naruto, he would not give up easily. He would try to prove his innocence and show them that they are wrong. He could go on a journey to find evidence or seek help from other allies who still believe in him, like Shikamaru or Kakashi.
One possible plot could be that Tsunade is misled by false information about Naruto. Maybe some powerful enemy fabricates evidence that Naruto is a threat to the village. Tsunade, believing this, exiles Naruto. Naruto, feeling betrayed, then has to prove his innocence while also facing external threats.
In some fanfictions, Tsunade might betray Naruto due to political pressure. Maybe the village elders force her hand, believing Naruto's power is too dangerous. It could also be a misunderstanding, like false information about Naruto being a threat to the village reaching Tsunade.
In the fanfiction, it might be that Tsunade believes Naruto has become too dangerous. Maybe she's misled by false information about Naruto's new powers being uncontrollable and a threat to the village. So she decides to betray him to protect Konoha from a potential disaster.
Perhaps a plot twist would be that there is some sort of curse associated with Naruto's lineage as their grandson. This curse could be related to the same forces that Tsunade and Jiraiya fought against in their lives. Naruto has to struggle not only with his normal ninja duties but also with this hidden curse. It could manifest in strange ways, like affecting his chakra control or making him a target for certain enemies. On the other hand, it could also be a source of great strength if he can overcome it. And there could be a prophecy related to him being the grandson of Tsunade and Jiraiya that he has to fulfill, which adds another layer of complexity to the story.